![](/images/graphics-bg.png)
Imbalance of the Nerve Growth Factor and Its Precursor as a Potential Biomarker for Diabetic Retinopathy
Joint Authors
Mysona, B. A.
Matragoon, S.
Stephens, M.
Mohamed, I. N.
Farooq, A.
Bartasis, M. L.
Fouda, A. Y.
Shanab, A. Y.
Espinosa-Heidmann, D. G.
El-Remessy, Azza B.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Our previous studies have demonstrated that diabetes-induced oxidative stress alters homeostasis of retinal nerve growth factor (NGF) resulting in accumulation of its precursor, proNGF, at the expense of NGF which plays a critical role in preserving neuronal and retinal function.
This imbalance coincided with retinal damage in experimental diabetes.
Here we test the hypothesis that alteration of proNGF and NGF levels observed in retina and vitreous will be mirrored in serum of diabetic patients.
Blood and vitreous samples were collected from patients (diabetic and nondiabetic) undergoing vitrectomy at Georgia Regents University under approved IRB.
Levels of proNGF, NGF, and p75NTR shedding were detected using Western blot analysis.
MMP-7 activity was also assayed.
Diabetes-induced proNGF expression and impaired NGF expression were observed in vitreous and serum.
Vitreous and sera from diabetic patients (n=11) showed significant 40.8-fold and 3.6-fold increases, respectively, compared to nondiabetics (n=9).
In contrast, vitreous and sera from diabetic patients showed significant 44% and 64% reductions in NGF levels, respectively, compared to nondiabetics.
ProNGF to NGF ratios showed significant correlation between vitreous and serum.
Further characterization of diabetes-induced imbalance in the proNGF to NGF ratio will facilitate its utility as an early biomarker for diabetic complications.
American Psychological Association (APA)
Mysona, B. A.& Matragoon, S.& Stephens, M.& Mohamed, I. N.& Farooq, A.& Bartasis, M. L.…[et al.]. 2015. Imbalance of the Nerve Growth Factor and Its Precursor as a Potential Biomarker for Diabetic Retinopathy. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055956
Modern Language Association (MLA)
Mysona, B. A.…[et al.]. Imbalance of the Nerve Growth Factor and Its Precursor as a Potential Biomarker for Diabetic Retinopathy. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055956
American Medical Association (AMA)
Mysona, B. A.& Matragoon, S.& Stephens, M.& Mohamed, I. N.& Farooq, A.& Bartasis, M. L.…[et al.]. Imbalance of the Nerve Growth Factor and Its Precursor as a Potential Biomarker for Diabetic Retinopathy. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055956