![](/images/graphics-bg.png)
Mass Spectral Profile for Rapid Differentiating Beta-Lactams from Their Ring-Opened Impurities
Joint Authors
Wang, Hecheng
Huang, Haiwei
Cao, Jin
Chui, Dehua
Xiao, Shengyuan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
High performance liquid chromatography tandem mass spectrometry (HPLC MS) has been widely used for β-lactam antibiotics determination.
However, its application to identify impurities of these frequently used drugs is not sufficient at present.
In this job, characteristic profiles of the collision induced dissociation (CID) spectra of both β-lactams and ring-opened β-lactams were extracted from the MS data of six β-lactam antibiotics and their forty-five impurities, and were confirmed by the MS data reported in the literature.
These characteristics have been successfully applied to rapid differentiation of β-lactam and ring-opened β-lactam impurities in cefixime, cefdinir, and cefaclor.
However, these characteristic profiles can only be obtained under low activating voltage.
They did not display in the high energy activated CID spectra.
Diagnostic fragmentations for determining the localization of double bond and substituents on the thiazine ring and the side chain were also observed.
In addition, several characteristic fragmentations are hopeful to be used to differentiate the configurations of C-2 on the thiazine ring of ring-opened impurities, which is generally disadvantageous of mass spectrometry.
Taken together, forty-five impurities were identified from the capsules of cefixime, cefdinir, and cefaclor.
American Psychological Association (APA)
Wang, Hecheng& Huang, Haiwei& Cao, Jin& Chui, Dehua& Xiao, Shengyuan. 2015. Mass Spectral Profile for Rapid Differentiating Beta-Lactams from Their Ring-Opened Impurities. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1056423
Modern Language Association (MLA)
Wang, Hecheng…[et al.]. Mass Spectral Profile for Rapid Differentiating Beta-Lactams from Their Ring-Opened Impurities. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1056423
American Medical Association (AMA)
Wang, Hecheng& Huang, Haiwei& Cao, Jin& Chui, Dehua& Xiao, Shengyuan. Mass Spectral Profile for Rapid Differentiating Beta-Lactams from Their Ring-Opened Impurities. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1056423
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056423