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Cooperative Regulation of Substrate Stiffness and Extracellular Matrix Proteins in Skin Wound Healing of Axolotls
Joint Authors
Huang, Ting-Yu
Wu, Cheng-Han
Wang, Mu-Hui
Chen, Bo-Sung
Chiou, Ling-Ling
Lee, Hsuan-Shu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Urodele amphibians (Ambystoma mexicanum), unique among vertebrates, can regenerate appendages and other body parts entirely and functionally through a scar-free healing process.
The wound epithelium covering the amputated or damaged site forms early and is essential for initiating the subsequent regenerative steps.
However, the molecular mechanism through which the wound reepithelializes during regeneration remains unclear.
In this study, we developed an in vitro culture system that mimics an in vivo wound healing process; the biomechanical properties in the system were precisely defined and manipulated.
Skin explants that were cultured on 2 to 50 kPa collagen-coated substrates rapidly reepithelialized within 10 to 15 h; however, in harder (1 GPa) and other extracellular matrices (tenascin-, fibronectin-, and laminin-coated environments), the wound epithelium moved slowly.
Furthermore, the reepithelialization rate of skin explants from metamorphic axolotls cultured on a polystyrene plate (1 GPa) increased substantially.
These findings afford new insights and can facilitate investigating wound epithelium formation during early regeneration using biochemical and mechanical techniques.
American Psychological Association (APA)
Huang, Ting-Yu& Wu, Cheng-Han& Wang, Mu-Hui& Chen, Bo-Sung& Chiou, Ling-Ling& Lee, Hsuan-Shu. 2015. Cooperative Regulation of Substrate Stiffness and Extracellular Matrix Proteins in Skin Wound Healing of Axolotls. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056461
Modern Language Association (MLA)
Huang, Ting-Yu…[et al.]. Cooperative Regulation of Substrate Stiffness and Extracellular Matrix Proteins in Skin Wound Healing of Axolotls. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056461
American Medical Association (AMA)
Huang, Ting-Yu& Wu, Cheng-Han& Wang, Mu-Hui& Chen, Bo-Sung& Chiou, Ling-Ling& Lee, Hsuan-Shu. Cooperative Regulation of Substrate Stiffness and Extracellular Matrix Proteins in Skin Wound Healing of Axolotls. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056461