Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
Joint Authors
Baumgart, Mario
Groth, Marco
Marthandan, Shiva
Priebe, Steffen
Cellerino, Alessandro
Guthke, Reinhard
Hemmerich, Peter
Diekmann, Stephan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-03
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Replicative senescence is of fundamental importance for the process of cellular aging, since it is a property of most of our somatic cells.
Here, we elucidated this process by comparing gene expression changes, measured by RNA-seq, in fibroblasts originating from two different tissues, embryonic lung (MRC-5) and foreskin (HFF), at five different time points during their transition into senescence.
Although the expression patterns of both fibroblast cell lines can be clearly distinguished, the similar differential expression of an ensemble of genes was found to correlate well with their transition into senescence, with only a minority of genes being cell line specific.
Clustering-based approaches further revealed common signatures between the cell lines.
Investigation of the mRNA expression levels at various time points during the lifespan of either of the fibroblasts resulted in a number of monotonically up- and downregulated genes which clearly showed a novel strong link to aging and senescence related processes which might be functional.
In terms of expression profiles of differentially expressed genes with age, common genes identified here have the potential to rule the transition into senescence of embryonic lung and foreskin fibroblasts irrespective of their different cellular origin.
American Psychological Association (APA)
Marthandan, Shiva& Priebe, Steffen& Baumgart, Mario& Groth, Marco& Cellerino, Alessandro& Guthke, Reinhard…[et al.]. 2015. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. BioMed Research International،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1056552
Modern Language Association (MLA)
Marthandan, Shiva…[et al.]. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. BioMed Research International No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1056552
American Medical Association (AMA)
Marthandan, Shiva& Priebe, Steffen& Baumgart, Mario& Groth, Marco& Cellerino, Alessandro& Guthke, Reinhard…[et al.]. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1056552
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056552