Surgical Modification of the Murine Calvaria Osteolysis Model

Joint Authors

Al-quhali, Ali Mohammed
Sun, Yu
Bai, Xizhuang
Jin, Zhe
Yu, Guibo

Source

BioMed Research International

Issue

Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-12-03

Country of Publication

Egypt

No. of Pages

5

Main Subjects

Medicine

Abstract EN

The murine calvaria model has been adopted for evaluation of osteolysis and inflammation induced by polyethylene (PE) or metal wear debris.

However, this model suffers from several complications.

The purpose of our study is to introduce a surgical modification with lower complication rates, thus providing more accurate results.

Forty C57/BL6 mice were divided into two groups, both receiving polyethylene particles.

Surgical modifications were performed in group 1, and group 2 underwent traditional surgeries.

The incidence of fluid leakage was recorded on the operative day.

Curst formation, wound dehiscence, and bone exposure were recorded on day 7.

Histological osteolysis was demonstrated by HE staining of tissue slices.

Micro-CT was used for quantifying evaluation of osteolysis in two groups.

Intraoperative fluid leakage was significantly reduced in group 1.

Postoperative crust formation, wound dehiscence, and bone exposure were also significantly decreased in group 1.

HE staining results revealed obvious osteolysis in group 1 and more obvious osteolysis in group 2.

Bone volume fraction (BVF) was (0.32 ± 0.03) in group 1 compared to group 2 (0.24 ± 0.05).

Bone mineral density (BMD) was (1.11 ± 0.03) in group 1 compared to group 2 (1.01 ± 0.02).

Surgical modifications provide a reliable way for establishment of the murine calvaria osteolysis model.

American Psychological Association (APA)

Al-quhali, Ali Mohammed& Sun, Yu& Bai, Xizhuang& Jin, Zhe& Yu, Guibo. 2015. Surgical Modification of the Murine Calvaria Osteolysis Model. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1056781

Modern Language Association (MLA)

Al-quhali, Ali Mohammed…[et al.]. Surgical Modification of the Murine Calvaria Osteolysis Model. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1056781

American Medical Association (AMA)

Al-quhali, Ali Mohammed& Sun, Yu& Bai, Xizhuang& Jin, Zhe& Yu, Guibo. Surgical Modification of the Murine Calvaria Osteolysis Model. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1056781

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1056781