Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 12 and Hepatitis C Virus Using Sol-Gel Based Microarray
Joint Authors
Lim, Chae Seung
Yun, Seung Gyu
Jang, Jin Woo
Lee, Jong Han
Kim, Jinhong
Ki, Yeona
Jo, Minjoung
Kim, Soyoun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Microarrays enable high-throughput screening (HTS) of disease-related molecules, including important signaling proteins/peptides and small molecules that are in low abundance.
In this study, we developed a multiplex blood bank screening platform, referred to as the Hi3-1 assay, for simultaneous detection of human immunodeficiency virus 1/2 (HIV 1/2) and hepatitis C virus (HCV).
Methods.
The Hi3-1 assay was tested using four panels (Panel 1, n=4,581 patient samples; Panel 2, n=15 seroconversion samples; Panel 3, n=4 performance samples; and Panel 4, n=251 purchased positive control samples), and the results were collected by the Department of Laboratory Medicine, Korea University Medical College, Republic of Korea.
The present study compares the sensitivity of the multiplex detection platform for both HIV and HCV using a sol-gel based microarray, which was based on a reference test (Architect HIV Ag/Ab Combo and Architect anti-HCV assays), in Korean patients.
Results.
The sensitivity of the multiplex detection platform for both HIV and HCV was 100%, and the specificity was 99.96% for HIV and 99.76% for HCV, which is equivalent to that of the reference test.
Conclusion.
We have successfully applied a novel screening technology to multiplex HIV and HCV diagnoses in a blood bank screening test.
American Psychological Association (APA)
Yun, Seung Gyu& Jang, Jin Woo& Lee, Jong Han& Lim, Chae Seung& Kim, Jinhong& Ki, Yeona…[et al.]. 2015. Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 12 and Hepatitis C Virus Using Sol-Gel Based Microarray. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056940
Modern Language Association (MLA)
Yun, Seung Gyu…[et al.]. Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 12 and Hepatitis C Virus Using Sol-Gel Based Microarray. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1056940
American Medical Association (AMA)
Yun, Seung Gyu& Jang, Jin Woo& Lee, Jong Han& Lim, Chae Seung& Kim, Jinhong& Ki, Yeona…[et al.]. Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 12 and Hepatitis C Virus Using Sol-Gel Based Microarray. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056940
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056940