Ghrelin Improves Functional Survival of Engrafted Adipose-Derived Mesenchymal Stem Cells in Ischemic Heart through PI3KAkt Signaling Pathway
Joint Authors
Ma, Sai
Han, Dong
Huang, Wei
Chen, Jiangwei
Gao, Lina
Liu, Tong
Zhang, Rongqing
Li, Congye
Fan, Miaomiao
Chen, Yundai
Cao, Feng
Li, Xiujuan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) have been proposed as a promising cell population for cell therapy and regenerative medicine applications.
However, the low retention and poor survival of engrafted cells hampered the therapeutic efficacy of engrafted MSCs.
Ghrelin is a 28-amino-acid peptide hormone and is proved to exert a protective effect on the cardiovascular system.
This study is designed to investigate the protective effects of ghrelin on engrafted adipose-derived mesenchymal stem cells (ADMSCs) and its beneficial effects with cellular therapy in mice myocardial infarction (MI).
Results showed that intramyocardial injection of ADMSCs combining with ghrelin administration inhibited host cardiomyocyte apoptosis, reduced fibrosis, and improved cardiac function.
To reveal possible mechanisms, ADMSCs were subjected to hypoxia/serum deprivation (H/SD) injury to simulate ischemic conditions in vivo.
Ghrelin (10−8 M, 33712 pg/ml) improved ADMSCs survival under H/SD condition.
Western blot assay revealed that ghrelin increasedAKT phosphorylation both in vivo and in vitro, decreased the proapoptotic protein Bax, and increased the antiapoptotic protein Bcl-2 in vitro, while these effects were abolished by PI3K inhibitor LY294002.
These revealed that ghrelin may serve as a promising candidate for hormone-driven approaches to improve the efficacy of mesenchymal stem cell-based therapy for cardiac ischemic disease via PI3K/AKT pathway.
American Psychological Association (APA)
Han, Dong& Huang, Wei& Ma, Sai& Chen, Jiangwei& Gao, Lina& Liu, Tong…[et al.]. 2015. Ghrelin Improves Functional Survival of Engrafted Adipose-Derived Mesenchymal Stem Cells in Ischemic Heart through PI3KAkt Signaling Pathway. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1057034
Modern Language Association (MLA)
Han, Dong…[et al.]. Ghrelin Improves Functional Survival of Engrafted Adipose-Derived Mesenchymal Stem Cells in Ischemic Heart through PI3KAkt Signaling Pathway. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1057034
American Medical Association (AMA)
Han, Dong& Huang, Wei& Ma, Sai& Chen, Jiangwei& Gao, Lina& Liu, Tong…[et al.]. Ghrelin Improves Functional Survival of Engrafted Adipose-Derived Mesenchymal Stem Cells in Ischemic Heart through PI3KAkt Signaling Pathway. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1057034
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057034