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Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta
Joint Authors
Ok, Seong-Ho
Shin, Il-Woo
Park, Jungchul
Yu, Jongsun
Lee, Youngju
Cho, Hyunhoo
Baik, Jiseok
Hong, Jeong-Min
Lee, Heon Keun
Chung, Young-Kyun
Sohn, Ju-Tae
Choi, Mun-Jeoung
Han, Jeong Yeol
Lee, Soo Hee
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We investigated the effect of Lipofundin MCT/LCT and Intralipid on acetylcholine-induced nitric oxide- (NO-) mediated relaxation in rat aorta to determine which lipid emulsion (LE) is more potent in terms of inhibition of NO-induced relaxation.
Dose-response curves of responses induced by acetylcholine, the calcium ionophore A23187, and sodium nitroprusside were generated using isolated rat aorta with or without LE.
The effect of Lipofundin MCT/LCT on acetylcholine-induced endothelial nitric oxide synthase (eNOS) phosphorylation in human umbilical vein endothelial cells (HUVECs) was investigated using western blotting.
Lipofundin MCT/LCT (0.1 and 0.2%) attenuated acetylcholine-induced relaxation in endothelium-intact aorta with or without tiron, whereas 0.2% Intralipid only inhibited relaxation.
Lipofundin MCT/LCT inhibited relaxation induced by the calcium ionophore A23187 and sodium nitroprusside in endothelium-intact aorta, but Lipofundin MCT/LCT had no effect on sodium nitroprusside-induced relaxation in the endothelium-denuded aorta.
Combined pretreatment with l-arginine plus Lipofundin MCT/LCT increased acetylcholine-induced maximal relaxation in endothelium-intact aorta compared with Lipofundin MCT/LCT alone.
l-Arginine attenuated Lipofundin MCT/LCT-mediated inhibition of acetylcholine-induced eNOS phosphorylation in HUVECs.
Taken together, Lipofundin MCT/LCT attenuated acetylcholine-induced NO-mediated relaxation via an inhibitory effect on the endothelium including eNOS, which is proximal to activation of guanylyl cyclase.
American Psychological Association (APA)
Ok, Seong-Ho& Lee, Soo Hee& Yu, Jongsun& Park, Jungchul& Shin, Il-Woo& Lee, Youngju…[et al.]. 2015. Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057088
Modern Language Association (MLA)
Ok, Seong-Ho…[et al.]. Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1057088
American Medical Association (AMA)
Ok, Seong-Ho& Lee, Soo Hee& Yu, Jongsun& Park, Jungchul& Shin, Il-Woo& Lee, Youngju…[et al.]. Lipid Emulsion Attenuates Acetylcholine-Induced Relaxation in Isolated Rat Aorta. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057088