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Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus
Joint Authors
Guo, Hongbo
Zhang, Yuejin
Chen, Yuanyuan
Wang, Ruihong
Zeng, Ailin
Deyholos, Michael K.
Shu, Jia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications.
The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively.
Mononucleotide was the most abundant type, followed by trinucleotide.
Among five families, Ganodermataceae occupied the most SSR markers, followed by Coriolaceae.
Functional prediction of SSR marker-containing EST sequences in Ganoderma lucidum obtained three main groups, namely, cellular component, biological process, and molecular function.
Thirty EST-SSR primers were designed to evaluate the genetic diversity of 13 natural Polyporus umbellatus accessions.
Twenty one EST-SSRs were polymorphic with average PIC value of 0.33 and transferability rate of 71%.
These 13 P.
umbellatus accessions showed relatively high genetic diversity.
The expected heterozygosity, Nei’s gene diversity, and Shannon information index were 0.41, 0.39, and 0.57, respectively.
Both UPGMA dendrogram and principal coordinate analysis (PCA) showed the same cluster result that divided the 13 accessions into three or four groups.
American Psychological Association (APA)
Zhang, Yuejin& Chen, Yuanyuan& Wang, Ruihong& Zeng, Ailin& Deyholos, Michael K.& Shu, Jia…[et al.]. 2015. Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1057321
Modern Language Association (MLA)
Zhang, Yuejin…[et al.]. Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1057321
American Medical Association (AMA)
Zhang, Yuejin& Chen, Yuanyuan& Wang, Ruihong& Zeng, Ailin& Deyholos, Michael K.& Shu, Jia…[et al.]. Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1057321
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057321