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Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability
Joint Authors
Chye, Joachim Loo Say
Mundargi, Raghavendra
Venkataraman, Divya
Kumar, Saranya
Mogal, Vishal
Ortiz, Raphael
Venkatraman, Subbu
Steele, Terry
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue.
Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow.
The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator “resorufin.” Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions.
These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels.
Histological evaluations with hematoxylin and eosin and Masson’s trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements.
Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.
American Psychological Association (APA)
Mundargi, Raghavendra& Venkataraman, Divya& Kumar, Saranya& Mogal, Vishal& Ortiz, Raphael& Chye, Joachim Loo Say…[et al.]. 2015. Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1057375
Modern Language Association (MLA)
Mundargi, Raghavendra…[et al.]. Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1057375
American Medical Association (AMA)
Mundargi, Raghavendra& Venkataraman, Divya& Kumar, Saranya& Mogal, Vishal& Ortiz, Raphael& Chye, Joachim Loo Say…[et al.]. Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1057375
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057375