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Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model
Joint Authors
Bridgewater, Laura C.
Olsen, Daniel S.
Goar, Wesley A.
Nichols, Brandt A.
Bailey, K. Tyson
Christensen, S. Loyd
Merriam, Kayla R.
Reynolds, Paul R.
Wilson, Eric
Weber, K. Scott
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We recently identified a nuclear variant of the BMP2 growth factor, called nBMP2.
In an effort to understand the function of this variant protein, we generated a mouse line in which BMP2 is expressed and functions normally, but nBMP2 is excluded from the nucleus.
This novel mutation allows the study of nBMP2 without compromising BMP2 function.
To determine whether nBMP2 plays a role in immune function, we performed a series of experiments in which we compared mouse survival, organ weights, immune cells numbers, and bacterial load in wild type and n B m p 2 N L S t m mice following primary and secondary challenges with Staphylococcus aureus.
Following primary challenge with S.
aureus, wild type and n B m p 2 N L S t m mice showed no differences in survival or bacterial load and generated similar numbers and types of leukocytes, although mutant spleens were smaller than wild type.
Secondary bacterial challenge with S.
aureus, however, produced differences in survival, with increased mortality seen in n B m p 2 N L S t m mice.
This increased mortality corresponded to higher levels of bacteremia in n B m p 2 N L S t m mice and to a reduced enlargement of mutant spleens in response to the secondary infection.
Together, these results suggest that the recently described nuclear variant of BMP2 is necessary for efficient secondary immune responses.
American Psychological Association (APA)
Olsen, Daniel S.& Goar, Wesley A.& Nichols, Brandt A.& Bailey, K. Tyson& Christensen, S. Loyd& Merriam, Kayla R.…[et al.]. 2015. Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1057458
Modern Language Association (MLA)
Olsen, Daniel S.…[et al.]. Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1057458
American Medical Association (AMA)
Olsen, Daniel S.& Goar, Wesley A.& Nichols, Brandt A.& Bailey, K. Tyson& Christensen, S. Loyd& Merriam, Kayla R.…[et al.]. Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1057458
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057458