Blockade of Aquaporin 1 Inhibits Proliferation, Motility, and Metastatic Potential of Mesothelioma In Vitro but not in an In Vivo Model
Joint Authors
Klebe, Sonja
Griggs, Kim
Cheng, Yuen
Driml, Jack
Henderson, Douglas W.
Reid, Glen
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Malignant mesothelioma (MM) is an aggressive tumor of the serosal membranes, mostly the pleura.
It is related to asbestos exposure and has a poor prognosis.
MM has a long latency period, and incidence is predicted to remain stable or increase until 2020.
Currently, no biomarkers for a specific targeted therapy are available.
Previously, we observed that expression of aquaporin 1 (AQP1) was an indicator of prognosis in two independent cohorts.
Here we determine whether AQP1 inhibition has therapeutic potential in the treatment of MM.
Methods.
Functional studies were performed with H226 cells and primary MM cells harvested from pleural effusions.
AQP1 expression and mesothelial phenotype was determined by immunohistochemistry.
AQP1 function was inhibited by a pharmacological blocker (AqB050) or AQP1-specific siRNA.
Cell proliferation, migration, and anchorage-independent cell growth were assessed.
A nude mouse heterotopic xenograft model of MM was utilised for the in vivo studies.
Results.
Inhibition of AQP1 significantly decreases cell proliferation, metastatic potential, and motility without inducing nonspecific cytotoxicity or increasing apoptosis.
In vivo blockade of AQP1 had no biologically significant effect on growth of established tumours.
Conclusions.
Targeted blockade of AQP1 restricts MM growth and migration in vitro.
Further work is warranted to fully evaluate treatment potential in vivo.
American Psychological Association (APA)
Klebe, Sonja& Griggs, Kim& Cheng, Yuen& Driml, Jack& Henderson, Douglas W.& Reid, Glen. 2015. Blockade of Aquaporin 1 Inhibits Proliferation, Motility, and Metastatic Potential of Mesothelioma In Vitro but not in an In Vivo Model. Disease Markers،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1060897
Modern Language Association (MLA)
Klebe, Sonja…[et al.]. Blockade of Aquaporin 1 Inhibits Proliferation, Motility, and Metastatic Potential of Mesothelioma In Vitro but not in an In Vivo Model. Disease Markers No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1060897
American Medical Association (AMA)
Klebe, Sonja& Griggs, Kim& Cheng, Yuen& Driml, Jack& Henderson, Douglas W.& Reid, Glen. Blockade of Aquaporin 1 Inhibits Proliferation, Motility, and Metastatic Potential of Mesothelioma In Vitro but not in an In Vivo Model. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1060897
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1060897