The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells
Joint Authors
Gao, Chao
Cui, Yu-Gui
Sun, Dalin
Jin, Baofang
Zhang, Xindong
Gu, Yalong
Zhao, Hongle
Cai, Bin
Gao, Li
Tang, Zhian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Yangjing Capsule (YC), an innovative Chinese medicine based on traditional prescription, promotes testosterone synthesis by upregulating the expression of steroidogenic enzymes.
Nur77 as a nuclear receptor is known to regulate the expression of many steroid synthetases.
This study aimed to explore the potential mechanisms by which YC regulates testosterone synthesis in Leydig cells.
Real-time PCR and Western blot analysis were employed to assess the expressions of steroidogenic enzymes and Nur77 after treating MLTC-1 cells with YC.
The luciferase reporter gene assay was performed to detect the activity of Nur77 gene promoter.
Also, the expressions of steroid synthases were detected after Nur77 gene was knocked down.
YC significantly stimulated Nur77 production and upregulated StAR and HSD3B expression, and this agrees with the activity of Nur77 gene promoter that was significantly enhanced by YC.
Interestingly, knockdown of Nur77 blocked the above YC’s effects and consequently inhibited testosterone synthesis in MLTC-1 cells.
YC promotes StAR and HSD3B expression and upregulates testosterone synthesis in Leydig cells, which is mediated by Nur77 pathway.
American Psychological Association (APA)
Gu, Yalong& Zhang, Xindong& Sun, Dalin& Zhao, Hongle& Cai, Bin& Gao, Chao…[et al.]. 2015. The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061470
Modern Language Association (MLA)
Gu, Yalong…[et al.]. The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061470
American Medical Association (AMA)
Gu, Yalong& Zhang, Xindong& Sun, Dalin& Zhao, Hongle& Cai, Bin& Gao, Chao…[et al.]. The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061470