Effect of Electroacupuncture on Cell Apoptosis and ERK Signal Pathway in the Hippocampus of Adult Rats with Cerebral Ischemia-Reperfusion
Joint Authors
Wu, Chunxiao
Wang, Jiao
Li, Chun
Zhou, Guoping
Xu, Xiuhong
Zhang, Xin
Lan, Xiao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
EA therapy is a traditional therapeutic approach for alleviation of cerebral I/R-induced brain injury.
We investigated the effect of EA on MCAO rat model to examine the mechanism of apoptosis in the rat hippocampus.
Methods.
200 male Sprague-Dawley rats were randomly divided into sham, I/R, EA, ERK inhibitor (PD), and ERK inhibitor+EA (PD+EA) groups.
Each group was subdivided into 5 groups according to different time points.
Locomotor behaviors were evaluated using neurological scales and morphological examination was performed using HE staining.
Apoptosis index of neural cells in local infarcted area was measured by TUNEL and p-ERK expression was detected using immunohistochemistry technique and western blot analysis.
Results.
Neurological deficit scores and neural apoptosis in the EA group were lower than I/R group at the same time points, respectively.
At different time points, p-ERK level was increased in the ischemic hippocampal CA1 in the EA group as compared to I/R group; the increased level was increased most at 1 day, 3 days, and 1 week (p<0.01).
Conclusion.
EA alleviates neurological deficit, reduces apoptosis index, and simultaneously upregulates the expression of p-ERK signal pathway in rats subjected to I/R injury.
American Psychological Association (APA)
Wu, Chunxiao& Wang, Jiao& Li, Chun& Zhou, Guoping& Xu, Xiuhong& Zhang, Xin…[et al.]. 2015. Effect of Electroacupuncture on Cell Apoptosis and ERK Signal Pathway in the Hippocampus of Adult Rats with Cerebral Ischemia-Reperfusion. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061479
Modern Language Association (MLA)
Wu, Chunxiao…[et al.]. Effect of Electroacupuncture on Cell Apoptosis and ERK Signal Pathway in the Hippocampus of Adult Rats with Cerebral Ischemia-Reperfusion. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1061479
American Medical Association (AMA)
Wu, Chunxiao& Wang, Jiao& Li, Chun& Zhou, Guoping& Xu, Xiuhong& Zhang, Xin…[et al.]. Effect of Electroacupuncture on Cell Apoptosis and ERK Signal Pathway in the Hippocampus of Adult Rats with Cerebral Ischemia-Reperfusion. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061479
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061479