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Neuroprotection of Sanhua Decoction against Focal Cerebral IschemiaReperfusion Injury in Rats through a Mechanism Targeting Aquaporin 4
Joint Authors
Li, Ji-huang
Li, Hui-qin
Liu, Ai-ju
Zheng, Guo-qing
Lu, Lin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Sanhua decoction (SHD) is a famous classic Chinese herbal prescription for ischemic stroke, and aquaporin 4 (AQP4) is reported to play a key role in ischemic brain edema.
This study aimed to investigate neuroprotection of SHD against focal cerebral ischemia/reperfusion (I/R) injury in rats and explore the hypothesis that AQP4 probably is the target of SHD neuroprotection against I/R rats.
Lentiviral-mediated AQP4-siRNA was inducted into adult male Sprague-Dawley rats via intracerebroventricular injection.
The focal cerebral ischemia/reperfusion model was established by occluding middle cerebral artery.
Neurological examinations were performed according to Longa Scale.
Brain water content, was determined by wet and dry weight measurement.
Western blot was adopted to test the AQP4 expression in ipsilateral hippocampus.
After the treatment, SHD alleviated neurological deficits, reduced brain water content and downregulated the expression of AQP4 at different time points following I/R injury.
Furthermore, neurobehavioral function and brain edema after I/R were significantly attenuated via downregulation of AQP4 expression when combined with AQP4-siRNA technology.
In conclusion, SHD exerted neuroprotection against focal cerebral I/R injury in rats mainly through a mechanism targeting AQP4.
American Psychological Association (APA)
Lu, Lin& Li, Hui-qin& Li, Ji-huang& Liu, Ai-ju& Zheng, Guo-qing. 2015. Neuroprotection of Sanhua Decoction against Focal Cerebral IschemiaReperfusion Injury in Rats through a Mechanism Targeting Aquaporin 4. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063351
Modern Language Association (MLA)
Lu, Lin…[et al.]. Neuroprotection of Sanhua Decoction against Focal Cerebral IschemiaReperfusion Injury in Rats through a Mechanism Targeting Aquaporin 4. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1063351
American Medical Association (AMA)
Lu, Lin& Li, Hui-qin& Li, Ji-huang& Liu, Ai-ju& Zheng, Guo-qing. Neuroprotection of Sanhua Decoction against Focal Cerebral IschemiaReperfusion Injury in Rats through a Mechanism Targeting Aquaporin 4. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063351
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063351