![](/images/graphics-bg.png)
Effect of Sterols Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells
Joint Authors
Bolaños-Carrillo, Mario Augusto
Ventura-Gallegos, Jose Luis
Saldivar-Jiménez, Arturo David
Zentella-Dehesa, Alejandro
Martínez-Vázquez, Mariano
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To explore the effect of peniocerol and macdougallin on HCT-15 and MCF-7 cells proliferation, cell cycle, apoptosis, and PARP cleavage.
Methods.
HCT-15 and MCF-7 cells were treated with various concentrations of peniocerol and macdougallin (10–80 μM) during 24 or 48 h.
Crystal Violet Assay was used to evaluate the inhibition effect.
Cell cycle regulation was examined by a propidium iodide method.
Cell apoptosis was detected through both Annexin–V FLUOS/PI double-labeled cytometry assays and Western blot was applied to assess PARP cleavage.
Results.
Peniocerol and macdougallin induced growth inhibition and apoptosis in vitro in a time- and dose-dependent manner.
Moreover, peniocerol and macdougallin induced arrest of cell cycle-dependent manner and increased the proportion of cells in G0/G1 phase.
PARP cleavage in HCT-15 and MCF-7 cells was induced by treatment with peniocerol and macdougallin after 36 hours.
Conclusions.
Our results showed that the mechanism of cytotoxicity displayed by peniocerol and macdougallin is related to cell cycle arrest and apoptosis in both cell lines.
This is a significant observation because it helps to understand the way some oxysterols isolated from Myrtillocactus geometrizans develop their biological activities against cancer cells.
American Psychological Association (APA)
Bolaños-Carrillo, Mario Augusto& Ventura-Gallegos, Jose Luis& Saldivar-Jiménez, Arturo David& Zentella-Dehesa, Alejandro& Martínez-Vázquez, Mariano. 2015. Effect of Sterols Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063355
Modern Language Association (MLA)
Bolaños-Carrillo, Mario Augusto…[et al.]. Effect of Sterols Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063355
American Medical Association (AMA)
Bolaños-Carrillo, Mario Augusto& Ventura-Gallegos, Jose Luis& Saldivar-Jiménez, Arturo David& Zentella-Dehesa, Alejandro& Martínez-Vázquez, Mariano. Effect of Sterols Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063355