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Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines
Joint Authors
Rahman, Heshu Sulaiman
Azizi, Susan
Tahir, Paridah Mohd
Chartrand, Max Stanley
Mohammad, Rosfarizan
Namvar, Farideh
Yeap, Swee Keong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aim of this study is to evaluate the in vitro cytotoxic activity and cellular effects of previously prepared ZnO-NPs on murine cancer cell lines using brown seaweed (Sargassum muticum) aqueous extract.
Treated cancer cells with ZnO-NPs for 72 hours demonstrated various levels of cytotoxicity based on calculated IC50 values using MTT assay as follows: 21.7 ± 1.3 μg/mL (4T1), 17.45 ± 1.1 μg/mL (CRL-1451), 11.75 ± 0.8 μg/mL (CT-26), and 5.6 ± 0.55 μg/mL (WEHI-3B), respectively.
On the other hand, ZnO-NPs treatments for 72 hours showed no toxicity against normal mouse fibroblast (3T3) cell line.
On the other hand, paclitaxel, which imposed an inhibitory effect on WEHI-3B cells with IC50 of 2.25 ± 0.4, 1.17 ± 0.5, and 1.6 ± 0.09 μg/mL after 24, 48, and 72 hours treatment, respectively, was used as positive control.
Furthermore, distinct morphological changes were found by utilizing fluorescent dyes; apoptotic population was increased via flowcytometry, while a cell cycle block and stimulation of apoptotic proteins were also observed.
Additionally, the present study showed that the caspase activations contributed to ZnO-NPs triggered apoptotic death in WEHI-3 cells.
Thus, the nature of biosynthesis and the therapeutic potential of ZnO-NPs could prepare the way for further research on the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer.
American Psychological Association (APA)
Namvar, Farideh& Rahman, Heshu Sulaiman& Mohammad, Rosfarizan& Azizi, Susan& Tahir, Paridah Mohd& Chartrand, Max Stanley…[et al.]. 2015. Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063357
Modern Language Association (MLA)
Namvar, Farideh…[et al.]. Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1063357
American Medical Association (AMA)
Namvar, Farideh& Rahman, Heshu Sulaiman& Mohammad, Rosfarizan& Azizi, Susan& Tahir, Paridah Mohd& Chartrand, Max Stanley…[et al.]. Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063357
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063357