Anticancer Activity of Saponins from Allium chinense against the B16 Melanoma and 4T1 Breast Carcinoma Cell
Joint Authors
Xia, Liqiu
Yu, Zhihui
Zhang, Tong
Zhou, Fengjuan
Xiao, Xiuqing
He, Hao
Rang, Jie
Quan, Meifang
Wang, Ting
Zuo, Mingxing
Ding, Xuezhi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The cytotoxic substance of A.
chinense saponins (ACSs) was isolated using ethanol extraction and purified with the D101 macroporous adsorption resin approach.
We investigated the anticancer activity of ACSs in the B16 melanoma and 4T1 breast carcinoma cell lines.
Methylthioninium chloride and hematoxylin-eosin staining with Giemsa dyestuff were used when the cells were treated with ACSs.
The results showed that the cells morphologies changed significantly; ACSs induced cell death in B16 and 4T1 cells based on acridine orange/ethidium bromide double fluorescence staining, with the number and degree of apoptotic tumor cells increasing as ACS concentration increased.
ACSs inhibited the proliferation of B16 and 4T1 cells in a dose-dependent manner.
They also inhibited cell migration and colony formation and exhibited a concentration-dependent effect.
In addition, ACSs apparently inhibited the growth of melanoma in vivo.
The preliminary antitumor in vivo assay revealed that early medication positively affected tumor inhibition action and effectively protected the liver and spleen of C57 BL/6 mice from injury.
This study provides evidence for the cytotoxicity of ACSs and a strong foundation for further research to establish the theoretical basis for cell death and help in the design and development of new anticancer drugs.
American Psychological Association (APA)
Yu, Zhihui& Zhang, Tong& Zhou, Fengjuan& Xiao, Xiuqing& Ding, Xuezhi& He, Hao…[et al.]. 2015. Anticancer Activity of Saponins from Allium chinense against the B16 Melanoma and 4T1 Breast Carcinoma Cell. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063499
Modern Language Association (MLA)
Yu, Zhihui…[et al.]. Anticancer Activity of Saponins from Allium chinense against the B16 Melanoma and 4T1 Breast Carcinoma Cell. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1063499
American Medical Association (AMA)
Yu, Zhihui& Zhang, Tong& Zhou, Fengjuan& Xiao, Xiuqing& Ding, Xuezhi& He, Hao…[et al.]. Anticancer Activity of Saponins from Allium chinense against the B16 Melanoma and 4T1 Breast Carcinoma Cell. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063499
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063499