α-Mangostin Improves Glucose Uptake and Inhibits Adipocytes Differentiation in 3T3-L1 Cells via PPARγ, GLUT4, and Leptin Expressions
Joint Authors
Susanti, D.
Zakaria, Zainul Amiruddin
Taher, Muhammad
Mohamed Amiroudine, Mohamed Zaffar Ali
Tengku Zakaria, Tengku Muhamad Faris Syafiq
Ichwan, Solachuddin J. A.
Kaderi, Mohd Arifin
Ahmed, Qamar Uddin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer.
The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes.
In this study, we investigated the activity of α-mangostin, a major xanthone component isolated from the stem bark of G.
malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPARγ, GLUT4, and leptin expressions.
α-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation.
Cells treated with 50 μM of α-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells.
Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake ( P < 0.05 ) with highest activity found at 25 μM.
In addition, α-mangostin increased the amount of free fatty acids (FFA) released.
The highest glycerol release level was observed at 50 μM of α-mangostin.
qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPARγ gene expression.
Induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin.
These evidences propose that α-mangostin might be possible candidate for the effective management of obesity in future.
American Psychological Association (APA)
Taher, Muhammad& Mohamed Amiroudine, Mohamed Zaffar Ali& Tengku Zakaria, Tengku Muhamad Faris Syafiq& Susanti, D.& Ichwan, Solachuddin J. A.& Kaderi, Mohd Arifin…[et al.]. 2015. α-Mangostin Improves Glucose Uptake and Inhibits Adipocytes Differentiation in 3T3-L1 Cells via PPARγ, GLUT4, and Leptin Expressions. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063540
Modern Language Association (MLA)
Taher, Muhammad…[et al.]. α-Mangostin Improves Glucose Uptake and Inhibits Adipocytes Differentiation in 3T3-L1 Cells via PPARγ, GLUT4, and Leptin Expressions. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063540
American Medical Association (AMA)
Taher, Muhammad& Mohamed Amiroudine, Mohamed Zaffar Ali& Tengku Zakaria, Tengku Muhamad Faris Syafiq& Susanti, D.& Ichwan, Solachuddin J. A.& Kaderi, Mohd Arifin…[et al.]. α-Mangostin Improves Glucose Uptake and Inhibits Adipocytes Differentiation in 3T3-L1 Cells via PPARγ, GLUT4, and Leptin Expressions. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063540
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063540