Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis
Joint Authors
Li, Qi-hui
Xie, Wen-xia
Li, Xiao-pei
Huang, Ka-te
Du, Zhong-heng
Cong, Wen-jie
Zhou, Long-hua
Ye, Tian-shen
Chen, Jiang-Fan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To study the role of adenosine A2A receptor (A2AR) in mediating the anti-inflammatory effect of electroacupuncture (EA) on synovitis in collagen-induced arthritis (CIA), C57BL/6 mice were divided into five treatment groups: Sham-control, CIA-control, CIA-EA, CIA-SCH58261 (A2AR antagonist), and CIA-EA-SCH58261.
All mice except those in the Sham-control group were immunized with collagen II for arthritis induction.
EA treatment was administered using the stomach 36 and spleen 6 points, and stimulated with a continuous rectangular wave for 30 min daily.
EA treatment and SCH58261 were administered daily from days 35 to 49 (n=10).
After treatment, X-ray radiography of joint bone morphology was established at day 60 and mouse blood was collected for ELISA determination of tumor necrosis factor alpha (TNF-α) levels.
Mice were sacrificed and processed for histological examination of pathological changes of joint tissue, including hematoxylin-eosin staining and immunohistochemistry of A2AR expression.
EA treatment resulted in significantly reduced pathological scores, TNF-α concentrations, and bone damage X-ray scores.
Importantly, the anti-inflammatory and tissue-protective effect of EA treatment was reversed by coadministration of SCH58261.
Thus, EA treatment exerts an anti-inflammatory effect resulting in significant protection of cartilage by activation of A2AR in the synovial tissue of CIA.
American Psychological Association (APA)
Li, Qi-hui& Xie, Wen-xia& Li, Xiao-pei& Huang, Ka-te& Du, Zhong-heng& Cong, Wen-jie…[et al.]. 2015. Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063618
Modern Language Association (MLA)
Li, Qi-hui…[et al.]. Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1063618
American Medical Association (AMA)
Li, Qi-hui& Xie, Wen-xia& Li, Xiao-pei& Huang, Ka-te& Du, Zhong-heng& Cong, Wen-jie…[et al.]. Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063618