Effects of Abnormal Savda Munzip on the Proliferation Activity and Migration Ability of Fibroblasts Derived from Hypertrophic Scar In Vitro
Joint Authors
Wang, Hujun
Gao, Weicheng
Kong, Menglong
Li, Nan
Shaolin, Ma
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
To explore the effect of ASMq on proliferation and migration ability of the fibroblast derived from HS of donor (HSFbs) in vitro.
Methods.
The HSFbs were cultured from tissue specimens and passaged to the 3~4 generation, which were treated with the different concentrations of ASMq and 5-Fu from 1 to 11 days.
The difference of HSFbs proliferation activity was analyzed by the CCK-8 method.
The HSFbs migration ability in ASMq (0.4 mg/mL) was analyzed by the Cell Scratch method.
Results.
Transmission electron microscope result shows ASMq concentration significantly increases and fibroblast cell structure markedly change in the experimental group.
The proliferation activity of the HSFbs was obviously weakened in ASMq groups than those of the group A ( P < 0.05 ) at seven days.
The group C (0.4 mg/mL) is better suitable than other three ASMq treatment groups.
Cell Migration Assay shows that the migration ability HSFbs was significantly reduced in ASMq (0.4 mg/mL) treatment group compared with those of blank control group at both 24 h and 48 h ( P < 0.05 ).
Conclusions.
These results suggest that ASMq effectively restrains the proliferation and migration ability of the HTSFbs in vitro, which can be one of the mechanisms for the prevention and treatment of HS.
American Psychological Association (APA)
Wang, Hujun& Gao, Weicheng& Kong, Menglong& Li, Nan& Shaolin, Ma. 2015. Effects of Abnormal Savda Munzip on the Proliferation Activity and Migration Ability of Fibroblasts Derived from Hypertrophic Scar In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1063706
Modern Language Association (MLA)
Wang, Hujun…[et al.]. Effects of Abnormal Savda Munzip on the Proliferation Activity and Migration Ability of Fibroblasts Derived from Hypertrophic Scar In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1063706
American Medical Association (AMA)
Wang, Hujun& Gao, Weicheng& Kong, Menglong& Li, Nan& Shaolin, Ma. Effects of Abnormal Savda Munzip on the Proliferation Activity and Migration Ability of Fibroblasts Derived from Hypertrophic Scar In Vitro. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1063706
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063706