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Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK12 in 3T3-L1 Cells
Joint Authors
Shin, Hyeun-Kyoo
Seo, Chang-Seob
Yoo, Sae-Rom
Jeong, Soo-Jin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Adipogenesis is the cell differentiation process from preadipocytes into adipocytes and the critical action in the development of obesity.
In the present study, we conducted in vitro analyses to investigate the inhibitory effects of Samsoeum (SSE), a traditional herbal decoction.
SSE had no significant cytotoxic effect against either the undifferentiated or differentiated 3T3-L1 cells.
Oil Red O staining results showed that SSE significantly inhibited fat accumulation in adipocytes.
SSE treatment consistently reduced the intracellular triglyceride content in the cells.
SSE significantly inactivated glycerol-3-phosphate dehydrogenase (GPDH), a major link between carbohydrate and lipid metabolisms in 3T3-L1 adipocytes, and markedly inhibited the production of leptin, an important adipokine, in differentiated cells.
SSE markedly suppressed the mRNA expression of the adipogenesis-related genes peroxisome proliferator-activated receptor-gamma (PPAR-γ), CCAAT/enhancer binding protein-alpha (C/EBP-α), fatty acid synthase (FAS), lipoprotein lipase (LPL), and fatty acid binding protein 4 (FABP4).
Importantly, SSE increased the phosphorylation of ERK1/2, but not p38 MAPK and JNK, in adipose cells.
Overall, our results indicate that SSE exerts antiadipogenic activity and modulates expressions of adipogenesis-related genes and ERK1/2 activation in adipocytes.
American Psychological Association (APA)
Jeong, Soo-Jin& Yoo, Sae-Rom& Seo, Chang-Seob& Shin, Hyeun-Kyoo. 2015. Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK12 in 3T3-L1 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063726
Modern Language Association (MLA)
Jeong, Soo-Jin…[et al.]. Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK12 in 3T3-L1 Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1063726
American Medical Association (AMA)
Jeong, Soo-Jin& Yoo, Sae-Rom& Seo, Chang-Seob& Shin, Hyeun-Kyoo. Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK12 in 3T3-L1 Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063726
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063726