Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular Carcinoma
Joint Authors
Qin, Shukui
Ye, Qing
Liu, Yanhong
Feng, Jundong
Wu, Qiong
Qu, Wenshu
Yin, Xiaojin
Source
Gastroenterology Research and Practice
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To investigate the effect of endostar on specific angiogenesis induced by human hepatocellular carcinoma, this research systematically elucidated the inhibitory effect on HepG2-induced angiogenesis by endostar from 50 ng/mL to 50000 ng/mL.
We employed fluorescence quantitative Boyden chamber analysis, wound-healing assay, flow cytometry examination using a coculture system, quantitative analysis of tube formation, and in vivo Matrigel plug assay induced by HCC conditioned media (HCM) and HepG2 compared with normal hepatocyte conditioned media (NCM) and L02.
Then, we found that endostar as a tumor angiogenesis inhibitor could potently inhibit human umbilical vein endothelial cell (HUVEC) migration in response to HCM after four- to six-hour action, inhibit HCM-induced HUVEC migration to the lesion part in a dose-dependent manner between 50 ng/mL and 5000 ng/mL at 24 hours, and reduce HUVEC proliferation in a dose-dependent fashion.
Endostar inhibited HepG2-induced tube formation of HUVECs which peaked at 50 ng/mL.
In vivo Matrigel plug formation was also significantly reduced by endostar in HepG2 inducing system rather than in L02 inducing system.
It could be concluded that, at cell level, endostar inhibited the angiogenesis-related biological behaviors of HUVEC in response to HCC, including migration, adhesion proliferation, and tube formation.
At animal level, endostar inhibited the angiogenesis in response to HCC in Matrigel matrix.
American Psychological Association (APA)
Ye, Qing& Qin, Shukui& Feng, Jundong& Wu, Qiong& Qu, Wenshu& Yin, Xiaojin…[et al.]. 2015. Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular Carcinoma. Gastroenterology Research and Practice،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1064364
Modern Language Association (MLA)
Feng, Jundong…[et al.]. Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular Carcinoma. Gastroenterology Research and Practice No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1064364
American Medical Association (AMA)
Ye, Qing& Qin, Shukui& Feng, Jundong& Wu, Qiong& Qu, Wenshu& Yin, Xiaojin…[et al.]. Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular Carcinoma. Gastroenterology Research and Practice. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1064364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064364