A Rapid and Accurate Extraction Procedure for Analysing Free Amino Acids in Meat Samples by GC-MS
Joint Authors
Pérez-Palacios, Trinidad
Barroso, Miguel A.
Ruiz, Jorge
Antequera, Teresa
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study evaluated the use of a mixer mill as the homogenization tool for the extraction of free amino acids in meat samples, with the main goal of analyzing a large number of samples in the shortest time and minimizing sample amount and solvent volume.
Ground samples (0.2 g) were mixed with 1.5 mL HCl 0.1 M and homogenized in the mixer mill.
The final biphasic system was separated by centrifugation.
The supernatant was deproteinized, derivatized and analyzed by gas chromatography.
This procedure showed a high extracting ability, especially in samples with high free amino acid content (recovery = 88.73–104.94%).
It also showed a low limit of detection and quantification (3.8 · 10−4–6.6 · 10−4 μg μL−1 and 1.3 · 10−3–2.2 · 10−2 μg μL−1, resp.) for most amino acids, an adequate precision (2.15–20.15% for run-to-run), and a linear response for all amino acids (R 2 = 0.741–0.998) in the range of 1–100 µg mL−1.
Moreover, it takes less time and requires lower amount of sample and solvent than conventional techniques.
Thus, this is a cost and time efficient tool for homogenizing in the extraction procedure of free amino acids from meat samples, being an adequate option for routine analysis.
American Psychological Association (APA)
Pérez-Palacios, Trinidad& Barroso, Miguel A.& Ruiz, Jorge& Antequera, Teresa. 2015. A Rapid and Accurate Extraction Procedure for Analysing Free Amino Acids in Meat Samples by GC-MS. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064409
Modern Language Association (MLA)
Pérez-Palacios, Trinidad…[et al.]. A Rapid and Accurate Extraction Procedure for Analysing Free Amino Acids in Meat Samples by GC-MS. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1064409
American Medical Association (AMA)
Pérez-Palacios, Trinidad& Barroso, Miguel A.& Ruiz, Jorge& Antequera, Teresa. A Rapid and Accurate Extraction Procedure for Analysing Free Amino Acids in Meat Samples by GC-MS. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064409