Development of a Rapid and Simple Method for Detection of Protein Contaminants in Carmine
Joint Authors
Nakayama, Norihisa
Ohtsu, Yutaka
Maezawa-Kase, Daisuke
Sano, Ken-Ichi
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Protein contaminants in carmine can cause dyspnea and anaphylactic reactions in users and consumers of products containing this pigment.
The method generally used for detection of proteins in carmine has low reproducibility and is time-consuming.
In this study, a rapid, simple, and highly reproducible method was developed for the detection of protein contaminants in carmine.
This method incorporates acidic protein denaturation conditions and ultrafiltration.
To prevent protein aggregation, sodium dodecyl sulfate containing gel electrophoresis running buffer was used for dispersing the carmine before filtration.
An ultrafiltration device was used to separate the protein contaminants from carminic acid in the carmine solution.
Two ultrafiltration devices were compared, and a cylindrical device containing a modified polyethersulfone membrane gave the best results.
The method had high reproducibility.
American Psychological Association (APA)
Nakayama, Norihisa& Ohtsu, Yutaka& Maezawa-Kase, Daisuke& Sano, Ken-Ichi. 2015. Development of a Rapid and Simple Method for Detection of Protein Contaminants in Carmine. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1064440
Modern Language Association (MLA)
Nakayama, Norihisa…[et al.]. Development of a Rapid and Simple Method for Detection of Protein Contaminants in Carmine. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1064440
American Medical Association (AMA)
Nakayama, Norihisa& Ohtsu, Yutaka& Maezawa-Kase, Daisuke& Sano, Ken-Ichi. Development of a Rapid and Simple Method for Detection of Protein Contaminants in Carmine. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1064440
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064440