Stability-Indicating HPLC Determination of Trandolapril in Bulk Drug and Pharmaceutical Dosage Forms
Joint Authors
Al-Hawash, Leena A.
Shakya, Ashok K.
Saleem, Maher L.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A rapid, simple, accurate, precise, economical, robust, and stability indicating reverse phase HPLC-PDA procedure has been developed and validated for the determination of trandolapril.
The trandolapril was separated isocratically on Hypersil-Gold C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of 50% acetonitrile and 50% water (containing 0.025% triethylamine, pH 3.0 ± 0.1 ), at 25 ± 2 °C.
Retention time of the drug was ~4.6 min.
The eluted compounds were monitored and identified at 210 nm.
The linearity of the method was excellent ( r 2 > 0.9999 ) over the concentration range of 1–24 μg/mL; the limit of detection (LOD) and limit of quantitation (LOQ) were 0.0566 μg/mL and 0.1715 μg/mL, respectively.
The overall precision was less than 2%.
Mean recovery of trandolapril was more than 99%; no interference was found from the component present in the preparation.
Stability studies indicate that the drug was stable to sunlight and UV light.
The drug gives 6 different oxidative products on exposure to hydrogen peroxide.
Slight degradation was observed in acidic condition.
Degradation was higher in the alkaline condition compared to other conditions.
The robustness of the method was studied using factorial design experiment.
American Psychological Association (APA)
Al-Hawash, Leena A.& Shakya, Ashok K.& Saleem, Maher L.. 2015. Stability-Indicating HPLC Determination of Trandolapril in Bulk Drug and Pharmaceutical Dosage Forms. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064447
Modern Language Association (MLA)
Al-Hawash, Leena A.…[et al.]. Stability-Indicating HPLC Determination of Trandolapril in Bulk Drug and Pharmaceutical Dosage Forms. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1064447
American Medical Association (AMA)
Al-Hawash, Leena A.& Shakya, Ashok K.& Saleem, Maher L.. Stability-Indicating HPLC Determination of Trandolapril in Bulk Drug and Pharmaceutical Dosage Forms. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064447
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064447