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Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis
Joint Authors
Latif, Farida
Luchetti, Andrea
Walsh, Diana
Rodger, Fay
Clark, Graeme
Martin, Tom
Irving, Richard
Sanna, Mario
Robledo, Mercedes
Neumann, Hartmut P. H.
Woodward, Emma R.
Abbs, Stephen
Martin, Howard
Yao, Masahiro
Maher, E. R.
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
At least 12 genes (FH, HIF2A, MAX, NF1, RET, SDHA, SDHB, SDHC, SDHD, SDHAF2, TMEM127, and VHL) have been implicated in inherited predisposition to phaeochromocytoma (PCC), paraganglioma (PGL), or head and neck paraganglioma (HNPGL) and a germline mutation may be detected in more than 30% of cases.
Knowledge of somatic mutations contributing to PCC/PGL/HNPGL pathogenesis has received less attention though mutations in HRAS, HIF2A, NF1, RET, and VHL have been reported.
To further elucidate the role of somatic mutation in PCC/PGL/HNPGL tumourigenesis, we employed a next generation sequencing strategy to analyse “mutation hotspots” in 50 human cancer genes.
Mutations were identified for HRAS (c.37G>C; p.G13R and c.182A>G; p.Q61R) in 7.1% (6/85); for BRAF (c.1799T>A; p.V600E) in 1.2% (1/85) of tumours; and for TP53 (c.1010G>A; p.R337H) in 2.35% (2/85) of cases.
Twenty-one tumours harboured mutations in inherited PCC/PGL/HNPGL genes and no HRAS, BRAF, or TP53 mutations occurred in this group.
Combining our data with previous reports of HRAS mutations in PCC/PGL we find that the mean frequency of HRAS/BRAF mutations in sporadic PCC/PGL is 8.9% (24/269) and in PCC/PGL with an inherited gene mutation 0% (0/148) suggesting that HRAS/BRAF mutations and inherited PCC/PGL genes mutations might be mutually exclusive.
We report the first evidence for BRAF mutations in the pathogenesis of PCC/PGL/HNPGL.
American Psychological Association (APA)
Luchetti, Andrea& Walsh, Diana& Rodger, Fay& Clark, Graeme& Martin, Tom& Irving, Richard…[et al.]. 2015. Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1065563
Modern Language Association (MLA)
Luchetti, Andrea…[et al.]. Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis. International Journal of Endocrinology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1065563
American Medical Association (AMA)
Luchetti, Andrea& Walsh, Diana& Rodger, Fay& Clark, Graeme& Martin, Tom& Irving, Richard…[et al.]. Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1065563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065563