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Expression of Intratumoral IGF-II Is Regulated by the Gene Imprinting Status in Triple Negative Breast Cancer from Vietnamese Patients
Joint Authors
Radhakrishnan, Vinodh Kumar
Hernandez, Lorraine Christine
Anderson, Kendra
Tan, Qianwei
De León, Marino
De León, Daisy D.
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
African American women suffer higher incidence and mortality of triple negative breast cancer (TNBC) than Caucasian women.
TNBC is very aggressive, causing the worst clinical outcome.
We previously demonstrated that tumors from these patients express high IGF-II and exhibit high activation of the IGF signaling pathways.
IGF-II gene expression is imprinted (monoallelic), promotes tumor progression, and metastasis and regulates Survivin, a TNBC prognostic marker.
Since BC mortality has increased among young Vietnamese women, we analyzed 48 (paired) TNBC samples from Vietnamese patients to assess IGF-II expression.
We analyzed all samples by qrtPCR for identification of IGF-II heterozygosity and to determine allelic expression of the IGF-II gene.
We also analyzed the tissues for proIGF-II and Survivin by RT-PCR and Western blotting.
A total of 28 samples displayed IGF-II heterozygosity of which 78% were biallelic.
Tumors with biallelic IGF-II gene expression exhibited the highest levels of proIGF-II and Survivin.
Although 100% of these tissues corresponding normal samples were biallelic, they expressed significantly lower levels of or no proIGF-II and Survivin.
Thus, IGF-II biallelic gene expression is differentially regulated in normal versus tumor tissues.
We propose that intratumoral proIGF-II is dependent on the IGF-II gene imprinting status and it will promote a more aggressive TNBC.
American Psychological Association (APA)
Radhakrishnan, Vinodh Kumar& Hernandez, Lorraine Christine& Anderson, Kendra& Tan, Qianwei& De León, Marino& De León, Daisy D.. 2015. Expression of Intratumoral IGF-II Is Regulated by the Gene Imprinting Status in Triple Negative Breast Cancer from Vietnamese Patients. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1065688
Modern Language Association (MLA)
Radhakrishnan, Vinodh Kumar…[et al.]. Expression of Intratumoral IGF-II Is Regulated by the Gene Imprinting Status in Triple Negative Breast Cancer from Vietnamese Patients. International Journal of Endocrinology No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1065688
American Medical Association (AMA)
Radhakrishnan, Vinodh Kumar& Hernandez, Lorraine Christine& Anderson, Kendra& Tan, Qianwei& De León, Marino& De León, Daisy D.. Expression of Intratumoral IGF-II Is Regulated by the Gene Imprinting Status in Triple Negative Breast Cancer from Vietnamese Patients. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1065688
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065688