Characterization of Olfactory Ensheathing Glial Cells Cultured on PolyurethanePolylactide Electrospun Nonwovens
Joint Authors
Grzesiak, Jakub
Fryczkowski, Ryszard
Lis, Anna
Szarek, Dariusz
Laska, Jadwiga
Marycz, Krzysztof
Source
International Journal of Polymer Science
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this research was to evaluate novel biomaterials for neural regeneration.
The investigated materials were composed of polyurethane (PU) and polylactide (PLDL) blended at three different w/w ratios, that is, 5/5, 6/4, and 8/2 of PU/PLDL.
Ultrathin fibrous scaffolds were prepared using electrospinning.
The scaffolds were investigated for their applicability for nerve regeneration by culturing rat olfactory ensheathing glial cells.
Cells were cultured on the materials for seven days, during which cellular morphology, phenotype, and metabolic activity were analysed.
SEM analysis of the fabricated fibrous scaffolds showed fibers of a diameter mainly lower than 600 μm with unimportant volume of protrusions situated along the fibers, with nonsignificant differences between all analysed materials.
Cells cultured on the materials showed differences in their morphology and metabolic activity, depending on the blend composition.
The most proper morphology, with numerous p75+ and GFAP+ cells present, was observed in the sample 6/4, whereas the highest metabolic activity was measured in the sample 5/5.
However, none of the investigated samples showed cytotoxicity or negatively influenced cellular morphology.
Therefore, the novel electrospun fibrous materials may be considered for regenerative medicine applications, and especially when contacting with highly sensitive nervous cells.
American Psychological Association (APA)
Grzesiak, Jakub& Fryczkowski, Ryszard& Lis, Anna& Szarek, Dariusz& Laska, Jadwiga& Marycz, Krzysztof. 2015. Characterization of Olfactory Ensheathing Glial Cells Cultured on PolyurethanePolylactide Electrospun Nonwovens. International Journal of Polymer Science،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1066861
Modern Language Association (MLA)
Grzesiak, Jakub…[et al.]. Characterization of Olfactory Ensheathing Glial Cells Cultured on PolyurethanePolylactide Electrospun Nonwovens. International Journal of Polymer Science No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1066861
American Medical Association (AMA)
Grzesiak, Jakub& Fryczkowski, Ryszard& Lis, Anna& Szarek, Dariusz& Laska, Jadwiga& Marycz, Krzysztof. Characterization of Olfactory Ensheathing Glial Cells Cultured on PolyurethanePolylactide Electrospun Nonwovens. International Journal of Polymer Science. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1066861
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066861