Single-Round Patterned DNA Library Microarray Aptamer Lead Identification
Joint Authors
Martin, Jennifer A.
Mirau, Peter A.
Chushak, Yaroslav
Chávez, Jorge L.
Naik, Rajesh R.
Hagen, Joshua A.
Kelley-Loughnane, Nancy
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A method for identifying an aptamer in a single round was developed using custom DNA microarrays containing computationally derived patterned libraries incorporating no information on the sequences of previously reported thrombin binding aptamers.
The DNA library was specifically designed to increase the probability of binding by enhancing structural complexity in a sequence-space confined environment, much like generating lead compounds in a combinatorial drug screening library.
The sequence demonstrating the highest fluorescence intensity upon target addition was confirmed to bind the target molecule thrombin with specificity by surface plasmon resonance, and a novel imino proton NMR/2D NOESY combination was used to screen the structure for G-quartet formation.
We propose that the lack of G-quartet structure in microarray-derived aptamers may highlight differences in binding mechanisms between surface-immobilized and solution based strategies.
This proof-of-principle study highlights the use of a computational driven methodology to create a DNA library rather than a SELEX based approach.
This work is beneficial to the biosensor field where aptamers selected by solution based evolution have proven challenging to retain binding function when immobilized on a surface.
American Psychological Association (APA)
Martin, Jennifer A.& Mirau, Peter A.& Chushak, Yaroslav& Chávez, Jorge L.& Naik, Rajesh R.& Hagen, Joshua A.…[et al.]. 2015. Single-Round Patterned DNA Library Microarray Aptamer Lead Identification. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067167
Modern Language Association (MLA)
Martin, Jennifer A.…[et al.]. Single-Round Patterned DNA Library Microarray Aptamer Lead Identification. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1067167
American Medical Association (AMA)
Martin, Jennifer A.& Mirau, Peter A.& Chushak, Yaroslav& Chávez, Jorge L.& Naik, Rajesh R.& Hagen, Joshua A.…[et al.]. Single-Round Patterned DNA Library Microarray Aptamer Lead Identification. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067167
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067167