Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression

Abstract EN

T2DM patients demonstrate reduced GLP-1 receptor (GLP-1R) expression in their gastric glands.

Whether induced T2DM and T1DM differently affect the gastric GLP-1R expression is not known.

This study assessed extrapancreatic GLP-1R system in glandular stomach of rodents with different types of experimental diabetes.

T2DM and T1DM were induced in Psammomys obesus (PO) by high-energy (HE) diet and by streptozotocin (STZ) in Sprague Dawly (SD) rats, respectively.

GLP-1R expression was determined in glandular stomach by RT PCR and immunohistomorphological analysis.

The mRNA expression and cellular association of the GLP-1R in principal glands were similar in control PO and SD rats.

However, nutrient and chemical induced diabetes resulted in opposite alterations of glandular GLP-1R expression.

Diabetic PO demonstrated increased GLP-1R mRNA expression, intensity of cellular GLP-1R immunostaining, and frequency of GLP-1R positive cells in the neck area of principal glands compared with controls.

In contrast, SD diabetic rats demonstrated decreased GLP-1 mRNA, cellular GLP-1R immunoreactivity, and frequency of GLP-1R immunoreactive cells in the neck area compared with controls.

In conclusion, nutrient and chemical induced experimental diabetes result in distinct opposite alterations of GLP-1R expression in glandular stomach.

These results suggest that induced T1DM and T2DM may differently modulate GLP-1R system in enteropancreatic axis.