![](/images/graphics-bg.png)
Exposure of Monocytes to Lipoarabinomannan Promotes Their Differentiation into Functionally and Phenotypically Immature Macrophages
Joint Authors
Chávez-Galán, Leslie
Ocaña-Guzmán, Ranferi
Torre-Bouscoulet, Luis
García-de-Alba, Carolina
Sada-Ovalle, Isabel
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-11
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Lipoarabinomannan (LAM) is a lipid virulence factor secreted by Mycobacterium tuberculosis (Mtb), the etiologic agent of tuberculosis.
LAM can be measured in the urine or serum of tuberculosis patients (TB-patients).
Circulating monocytes are the precursor cells of alveolar macrophages and might be exposed to LAM in patients with active TB.
We speculated that exposing monocytes to LAM could produce phenotypically and functionally immature macrophages.
To test our hypothesis, human monocytes were stimulated with LAM (24–120 hours) and various readouts were measured.
The study showed that when monocytes were exposed to LAM, the frequency of CD68+, CD33+, and CD86+ macrophages decreased, suggesting that monocyte differentiation into mature macrophages was affected.
Regarding functionality markers, TLR2+ and TLR4+ macrophages also decreased, but the percentage of MMR+ expression did not change.
LAM-exposed monocytes generated macrophages that were less efficient in producing proinflammatory cytokines such as TNF- α and IFN- γ ; however, their phagocytic capacity was not modified.
Taken together, these data indicate that LAM exposure influenced monocyte differentiation and produced poorly functional macrophages with a different phenotype.
These results may help us understand how mycobacteria can limit the quality of the innate and adaptive immune responses.
American Psychological Association (APA)
Chávez-Galán, Leslie& Ocaña-Guzmán, Ranferi& Torre-Bouscoulet, Luis& García-de-Alba, Carolina& Sada-Ovalle, Isabel. 2015. Exposure of Monocytes to Lipoarabinomannan Promotes Their Differentiation into Functionally and Phenotypically Immature Macrophages. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1068663
Modern Language Association (MLA)
Chávez-Galán, Leslie…[et al.]. Exposure of Monocytes to Lipoarabinomannan Promotes Their Differentiation into Functionally and Phenotypically Immature Macrophages. Journal of Immunology Research No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1068663
American Medical Association (AMA)
Chávez-Galán, Leslie& Ocaña-Guzmán, Ranferi& Torre-Bouscoulet, Luis& García-de-Alba, Carolina& Sada-Ovalle, Isabel. Exposure of Monocytes to Lipoarabinomannan Promotes Their Differentiation into Functionally and Phenotypically Immature Macrophages. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1068663
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068663