Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages
Joint Authors
Shannahan, Jonathan H.
Sowrirajan, Hari
Persaud, Indushekhar
Podila, Ramakrishna
Brown, Jared M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Macrophages are central to the development of atherosclerosis by absorbing lipids, promoting inflammation, and increasing plaque deposition.
Nanoparticles (NPs) are becoming increasingly common in biomedical applications thereby increasing exposure to the immune and vascular systems.
This project investigated the influence of NPs on macrophage function and specifically cholesterol uptake.
Macrophages were exposed to 20 nm silver NPs (AgNPs), 110 nm AgNPs, or 20 nm Fe3O4 NPs for 2 h and NP uptake, cytotoxicity, and subsequent uptake of fluorescently labeled cholesterol were assessed.
Macrophage uptake of NPs did not induce cytotoxicity at concentrations utilized (25 μg/mL); however, macrophage exposure to 20 nm AgNPs reduced subsequent uptake of cholesterol.
Further, we assessed the impact of a cholesterol-rich environment on macrophage function following NP exposure.
In these sets of experiments, macrophages internalized NPs, exhibited no cytotoxicity, and altered cholesterol uptake.
Alterations in the expression of scavenger receptor-B1 following NP exposure, which likely influences cholesterol uptake, were observed.
Overall, NPs alter cholesterol uptake, which may have implications in the progression of vascular or immune mediated diseases.
Therefore, for the safe development of NPs for biomedical applications, it is necessary to understand their impact on cellular function and biological interactions in underlying disease environments.
American Psychological Association (APA)
Shannahan, Jonathan H.& Sowrirajan, Hari& Persaud, Indushekhar& Podila, Ramakrishna& Brown, Jared M.. 2015. Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068740
Modern Language Association (MLA)
Shannahan, Jonathan H.…[et al.]. Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages. Journal of Nanomaterials No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1068740
American Medical Association (AMA)
Shannahan, Jonathan H.& Sowrirajan, Hari& Persaud, Indushekhar& Podila, Ramakrishna& Brown, Jared M.. Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068740