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Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry
Joint Authors
Lee, Jae-Sung
Kim, Sae-Wan
Jang, Eun-Yoon
Kang, Byoung-Ho
Lee, Sang-Won
Sai-Anand, Gopalan
Lee, Seung-Ha
Kwon, Dae-Hyuk
Kang, Shin-Won
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We propose a nanobiosensor to evaluate a lung cancer-specific biomarker.
The nanobiosensor is based on an anodic aluminum oxide (AAO) chip and functions on the principles of localized surface plasmon resonance (LSPR) and interferometry.
The pore-depth of the fabricated nanoporous AAO chip was 1 µm and was obtained using a two-step electrochemical anodization process.
The sensor chip is sensitive to the refractive index (RI) changes of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized on the gold-deposited surface of the AAO chip.
In order to confirm the effectiveness of the sensor, the antibodies were immobilized on the surface of the AAO chip, and the lung cancer-specific biomarker was applied atop of the immobilized-antibody layer using the self-assembled monolayer method.
The nanoporous AAO chip was used as a sensor system to detect serum amyloid A1, which is a lung cancer-specific biomarker.
The specific reaction of the antigen-antibody contributes to the change in the RI.
This in turn causes a shift in the resonance spectrum in the refractive interference pattern.
The limit of detection (LOD) was found to be 100 ag/mL and the biosensor had high sensitivity over a wide concentration range.
American Psychological Association (APA)
Lee, Jae-Sung& Kim, Sae-Wan& Jang, Eun-Yoon& Kang, Byoung-Ho& Lee, Sang-Won& Sai-Anand, Gopalan…[et al.]. 2015. Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068799
Modern Language Association (MLA)
Lee, Jae-Sung…[et al.]. Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry. Journal of Nanomaterials No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1068799
American Medical Association (AMA)
Lee, Jae-Sung& Kim, Sae-Wan& Jang, Eun-Yoon& Kang, Byoung-Ho& Lee, Sang-Won& Sai-Anand, Gopalan…[et al.]. Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068799
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068799