A Comparative Study of the Metabolic and Skeletal Response of C57BL6J and C57BL6N Mice in a Diet-Induced Model of Type 2 Diabetes
Joint Authors
Rendina-Ruedy, Elizabeth
Hembree, Kelsey D.
Sasaki, Angela
Davis, McKale R.
Lightfoot, Stan A.
Clarke, Stephen L.
Smith, Brenda J.
Lucas, Edralin A.
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Type 2 diabetes mellitus (T2DM) represents a complex clinical scenario of altered energy metabolism and increased fracture incidence.
The C57BL/6 mouse model of diet-induced obesity has been used to study the mechanisms by which altered glucose homeostasis affects bone mass and quality, but genetic variations in substrains of C57BL/6 may have confounded data interpretation.
This study investigated the long-term metabolic and skeletal consequences of two commonly used C57BL/6 substrains to a high fat (HF) diet.
Male C57BL/6J, C57BL/6N, and the negative control strain, C3H/HeJ, mice were fed a control or HF diet for 24 wks.
C57BL/6N mice on a HF diet demonstrated an increase in plasma insulin and blood glucose as early as 4 wk, whereas these responses were delayed in the C57BL/6J mice.
The C57BL/6N mice exhibited more severe hepatic steatosis and inflammation.
Only the C57BL/6N mice lost significant trabecular bone in response to the high fat diet.
The C3H/HeJ mice were protected from bone loss.
The data show that C57BL/6J and C57BL/6N mice differ in their metabolic and skeletal response when fed a HF diet.
These substrain differences should be considered when designing experiments and are likely to have implications on data interpretation and reproducibility.
American Psychological Association (APA)
Rendina-Ruedy, Elizabeth& Hembree, Kelsey D.& Sasaki, Angela& Davis, McKale R.& Lightfoot, Stan A.& Clarke, Stephen L.…[et al.]. 2015. A Comparative Study of the Metabolic and Skeletal Response of C57BL6J and C57BL6N Mice in a Diet-Induced Model of Type 2 Diabetes. Journal of Nutrition and Metabolism،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1069522
Modern Language Association (MLA)
Rendina-Ruedy, Elizabeth…[et al.]. A Comparative Study of the Metabolic and Skeletal Response of C57BL6J and C57BL6N Mice in a Diet-Induced Model of Type 2 Diabetes. Journal of Nutrition and Metabolism No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1069522
American Medical Association (AMA)
Rendina-Ruedy, Elizabeth& Hembree, Kelsey D.& Sasaki, Angela& Davis, McKale R.& Lightfoot, Stan A.& Clarke, Stephen L.…[et al.]. A Comparative Study of the Metabolic and Skeletal Response of C57BL6J and C57BL6N Mice in a Diet-Induced Model of Type 2 Diabetes. Journal of Nutrition and Metabolism. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1069522
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069522