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Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
Joint Authors
Tajbakhsh, Saeed
Taherkhani, Reza
Farshadpour, Fatemeh
Makvandi, Manoochehr
Rajabi Memari, Hamid
Samarbafzadeh, Ali Reza
Sharifi, Nasrin
Naeimi, Behrouz
Akbarzadeh, Samad
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection.
Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine.
Methods.
A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups.
Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups.
Results.
The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro.
These responses were significantly higher in the recovered group compared to the control group.
These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines.
Conclusion.
The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate.
However, further studies are required to evaluate this protein in vivo.
American Psychological Association (APA)
Taherkhani, Reza& Farshadpour, Fatemeh& Makvandi, Manoochehr& Rajabi Memari, Hamid& Samarbafzadeh, Ali Reza& Sharifi, Nasrin…[et al.]. 2015. Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection. Journal of Tropical Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1070408
Modern Language Association (MLA)
Taherkhani, Reza…[et al.]. Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection. Journal of Tropical Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1070408
American Medical Association (AMA)
Taherkhani, Reza& Farshadpour, Fatemeh& Makvandi, Manoochehr& Rajabi Memari, Hamid& Samarbafzadeh, Ali Reza& Sharifi, Nasrin…[et al.]. Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection. Journal of Tropical Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1070408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1070408