Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells
Joint Authors
Lin, Yee-Shin
Cheng, Yi-Lin
Wan, Shu-Wen
Ou, Yi-Dan
Yu, Chia-Yi
Tsai, Tsung-Ting
Tseng, Po-Chun
Lin, Chiou-Feng
Chen, Chia-Ling
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Infection with dengue virus (DENV) causes an increase in proinflammatory responses, such as nitric oxide (NO) generation and TNF-α expression; however, the molecular mechanism underlying this inflammatory activation remains undefined, although the activation of the transcription factor NF-κB is generally involved.
In addition to TNF-α production in DENV-infected murine macrophage RAW264.7 cells, inducible NO synthase was transcriptionally and posttranslationally elevated and accompanied by NO generation.
NF-κB is known to be activated by DENV infection.
Pharmacologically inhibiting NF-κB activation abolishes iNOS/NO biosynthesis and TNF-α production.
With inhibition, the potential role of NF-κB in oxidative signaling regulation was prevented during DENV infection.
Heat-inactivated DENV failed to cause the identified inflammatory responses.
Pharmacological inhibition of TLR3 partly decreased NF-κB activation; however, it effectively abolished inducible iNOS/NO biosynthesis but did not inhibit TNF-α production.
In contrast to TLR3, viral protein NS2B3 also independently contributed to NF-κB activation to regulate TNF-α production.
These results show the distinct pathways for NF-κB activation caused by DENV infection individually for the regulation of iNOS/NO and TNF-α expression.
American Psychological Association (APA)
Cheng, Yi-Lin& Lin, Yee-Shin& Chen, Chia-Ling& Wan, Shu-Wen& Ou, Yi-Dan& Yu, Chia-Yi…[et al.]. 2015. Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072222
Modern Language Association (MLA)
Cheng, Yi-Lin…[et al.]. Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells. Mediators of Inflammation No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1072222
American Medical Association (AMA)
Cheng, Yi-Lin& Lin, Yee-Shin& Chen, Chia-Ling& Wan, Shu-Wen& Ou, Yi-Dan& Yu, Chia-Yi…[et al.]. Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072222