Enterococcus faecium NCIMB 10415 Modulates Epithelial Integrity, Heat Shock Protein, and Proinflammatory Cytokine Response in Intestinal Cells
Joint Authors
Klingspor, Shanti
Bondzio, Angelika
Martens, Holger
Aschenbach, Jörg R.
Bratz, Katharina
Tedin, Karsten
Einspanier, Ralf
Lodemann, Ulrike
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Probiotics have shown positive effects on gastrointestinal diseases; they have barrier-modulating effects and change the inflammatory response towards pathogens in studies in vitro.
The aim of this investigation has been to examine the response of intestinal epithelial cells to Enterococcus faecium NCIMB 10415 (E.
faecium), a probiotic positively affecting diarrhea incidence in piglets, and two pathogenic Escherichia coli (E.
coli) strains, with specific focus on the probiotic modulation of the response to the pathogenic challenge.
Porcine (IPEC-J2) and human (Caco-2) intestinal cells were incubated without bacteria (control), with E.
faecium, with enteropathogenic (EPEC) or enterotoxigenic E.
coli (ETEC) each alone or in combination with E.
faecium.
The ETEC strain decreased transepithelial resistance (TER) and increased IL-8 mRNA and protein expression in both cell lines compared with control cells, an effect that could be prevented by pre- and coincubation with E.
faecium.
Similar effects were observed for the increased expression of heat shock protein 70 in Caco-2 cells.
When the cells were challenged by the EPEC strain, no such pattern of changes could be observed.
The reduced decrease in TER and the reduction of the proinflammatory and stress response of enterocytes following pathogenic challenge indicate the protective effect of the probiotic.
American Psychological Association (APA)
Klingspor, Shanti& Bondzio, Angelika& Martens, Holger& Aschenbach, Jörg R.& Bratz, Katharina& Tedin, Karsten…[et al.]. 2015. Enterococcus faecium NCIMB 10415 Modulates Epithelial Integrity, Heat Shock Protein, and Proinflammatory Cytokine Response in Intestinal Cells. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072241
Modern Language Association (MLA)
Klingspor, Shanti…[et al.]. Enterococcus faecium NCIMB 10415 Modulates Epithelial Integrity, Heat Shock Protein, and Proinflammatory Cytokine Response in Intestinal Cells. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072241
American Medical Association (AMA)
Klingspor, Shanti& Bondzio, Angelika& Martens, Holger& Aschenbach, Jörg R.& Bratz, Katharina& Tedin, Karsten…[et al.]. Enterococcus faecium NCIMB 10415 Modulates Epithelial Integrity, Heat Shock Protein, and Proinflammatory Cytokine Response in Intestinal Cells. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072241
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072241