Effect of TLR4MyD88 Signaling Pathway on Expression of IL-1 β and TNF- α in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide
Joint Authors
Lin, Xuefen
Kong, Jingjing
Wu, Qingting
Yang, Yingying
Ji, Ping
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Accumulating evidence from previous studies suggested that interleukin-1 (IL-1β) and tumor necrosis factor-α (TNF-α) play an important role in pathogenesis of temporomandibular disorders (TMD).
However, the cell surface receptors and the intracellular signal pathways leading to these cytokines expression are not fully understood.
In the current study, we investigated the roles of Toll-like receptor 4 (TLR4) and its adaptor myeloid differentiation factor 88 (MyD88) in the expression of IL-1β and TNF-α in synovial fibroblasts (SFs) separated from rat temporomandibular joint (TMJ) with lipopolysaccharide (LPS) stimulation.
The results showed that treatment with LPS could increase TLR4, MyD88, IL-1β, and TNF-α expression at both mRNA and protein levels.
In addition, increased expression of IL-1β and TNF-α could be blocked by treatment with TAK-242, a blocker of TLR4 signaling, and also by MyD88 inhibitory peptide (MIP).
These findings suggested that maybe TLR4/MyD88 signal transduction pathway participates in enhanced expression of IL-1 and TNF-α in patients with TMD.
The activation of TLR4/MyD88 signal transduction pathway which results in production of proinflammatory factors may play a role in the pathogenesis of TMD.
American Psychological Association (APA)
Lin, Xuefen& Kong, Jingjing& Wu, Qingting& Yang, Yingying& Ji, Ping. 2015. Effect of TLR4MyD88 Signaling Pathway on Expression of IL-1 β and TNF- α in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072251
Modern Language Association (MLA)
Lin, Xuefen…[et al.]. Effect of TLR4MyD88 Signaling Pathway on Expression of IL-1 β and TNF- α in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072251
American Medical Association (AMA)
Lin, Xuefen& Kong, Jingjing& Wu, Qingting& Yang, Yingying& Ji, Ping. Effect of TLR4MyD88 Signaling Pathway on Expression of IL-1 β and TNF- α in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072251