Effect of Helicobacter pylori Eradication on TLR2 and TLR4 Expression in Patients with Gastric Lesions
Joint Authors
Goloni-Bertollo, Eny Maria
Matos Biselli-Périco, Joice
Fucuta Pereira, Patrícia
Do Vale, Edla Polsinelli Bedin Mascarin
Acayaba, Ricardo
Leite, Kátia Ramos Moreira
Rossi, Ana Flávia Teixeira
Cadamuro, Aline Cristina Targa
Silva, Ana Elizabete
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
Helicobacter pylori (Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response.
Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression in H.
pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment.
Methods.
A total of 37 patients CG-Hp+ were evaluated.
The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry.
Results.
Before treatment both TLR2 and TLR4 mRNA in CG-Hp+ patients were slightly increased (TLR2 = 1.32; TLR4 = 1.26) in relation to Hp-negative normal gastric mucosa (P≤0.05).
After successful eradication therapy no significant change was observed (TLR2 = 1.47; TLR4 = 1.53; P>0.05).
In addition, the cagA and vacA bacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values of TLR2 and TLR4, both before and after treatment.
Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results.
Conclusion.
In conclusion, the expression of both TLR2 and TLR4 is increased in CG-Hp+ patients regardless of cagA and vacA status and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated.
American Psychological Association (APA)
Cadamuro, Aline Cristina Targa& Rossi, Ana Flávia Teixeira& Matos Biselli-Périco, Joice& Fucuta Pereira, Patrícia& Do Vale, Edla Polsinelli Bedin Mascarin& Acayaba, Ricardo…[et al.]. 2015. Effect of Helicobacter pylori Eradication on TLR2 and TLR4 Expression in Patients with Gastric Lesions. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072376
Modern Language Association (MLA)
Cadamuro, Aline Cristina Targa…[et al.]. Effect of Helicobacter pylori Eradication on TLR2 and TLR4 Expression in Patients with Gastric Lesions. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072376
American Medical Association (AMA)
Cadamuro, Aline Cristina Targa& Rossi, Ana Flávia Teixeira& Matos Biselli-Périco, Joice& Fucuta Pereira, Patrícia& Do Vale, Edla Polsinelli Bedin Mascarin& Acayaba, Ricardo…[et al.]. Effect of Helicobacter pylori Eradication on TLR2 and TLR4 Expression in Patients with Gastric Lesions. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072376
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072376