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Collagen Induced Arthritis in DBA1J Mice Associates with Oxylipin Changes in Plasma
Joint Authors
Vreeken, Rob J.
Harms, Amy C.
Hankemeier, Thomas
Berger, Ruud
He, Min
van Wijk, Eduard
Wang, Mei
Strassburg, Katrin
Schoeman, Johannes C.
van Wietmarschen, Herman
van der Greef, Jan
Kobayashi, Masaki
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA).
The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice.
DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group.
The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS).
Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM).
Both univariate and multivariate statistical analyses were applied.
The results in univariate Student’s t-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis.
The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice.
The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-κB because of insufficient PPAR-γ ligands.
American Psychological Association (APA)
He, Min& van Wijk, Eduard& Berger, Ruud& Wang, Mei& Strassburg, Katrin& Schoeman, Johannes C.…[et al.]. 2015. Collagen Induced Arthritis in DBA1J Mice Associates with Oxylipin Changes in Plasma. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072416
Modern Language Association (MLA)
He, Min…[et al.]. Collagen Induced Arthritis in DBA1J Mice Associates with Oxylipin Changes in Plasma. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072416
American Medical Association (AMA)
He, Min& van Wijk, Eduard& Berger, Ruud& Wang, Mei& Strassburg, Katrin& Schoeman, Johannes C.…[et al.]. Collagen Induced Arthritis in DBA1J Mice Associates with Oxylipin Changes in Plasma. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072416
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072416