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The X-Linked Inhibitor of Apoptosis Protein Inhibitor Embelin Suppresses Inflammation and Bone Erosion in Collagen Antibody Induced Arthritis Mice
Joint Authors
Crotti, Tania N.
Dharmapatni, Anak A. S. S. K.
Haynes, David R.
Cantley, Melissa D.
Marino, Victor
Perilli, Egon
Smith, Malcolm D.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
To investigate the effect of Embelin, an inhibitor of X-Linked Inhibitor of Apoptosis Protein (XIAP), on inflammation and bone erosion in a collagen antibody induced arthritis (CAIA) in mice.
Methods.
Four groups of mice ( n = 6 per group) were allocated: CAIA untreated mice, CAIA treated with Prednisolone (10 mg/kg/day), CAIA treated with low dose Embelin (30 mg/kg/day), and CAIA treated with high dose Embelin (50 mg/kg/day).
Joint inflammation was evaluated using clinical paw score and histological assessments.
Bone erosion was assessed using micro-CT, tartrate resistant acid phosphatase (TRAP) staining, and serum carboxy-terminal collagen crosslinks (CTX-1) ELISA.
Immunohistochemistry was used to detect XIAP protein.
TUNEL was performed to identify apoptotic cells.
Results.
Low dose, but not high dose Embelin, suppressed inflammation as reflected by lower paw scores ( P < 0.05 ) and lower histological scores for inflammation.
Low dose Embelin reduced serum CTX-1 ( P < 0.05 ) and demonstrated lower histological score and TRAP counting, and slightly higher bone volume as compared to CAIA untreated mice.
XIAP expression was not reduced but TUNEL positive cells were more abundant in Embelin treated CAIA mice.
Conclusion.
Low dose Embelin suppressed inflammation and serum CTX-1 in CAIA mice, indicating a potential use for Embelin to treat pathological bone loss.
American Psychological Association (APA)
Dharmapatni, Anak A. S. S. K.& Cantley, Melissa D.& Marino, Victor& Perilli, Egon& Crotti, Tania N.& Smith, Malcolm D.…[et al.]. 2015. The X-Linked Inhibitor of Apoptosis Protein Inhibitor Embelin Suppresses Inflammation and Bone Erosion in Collagen Antibody Induced Arthritis Mice. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072424
Modern Language Association (MLA)
Dharmapatni, Anak A. S. S. K.…[et al.]. The X-Linked Inhibitor of Apoptosis Protein Inhibitor Embelin Suppresses Inflammation and Bone Erosion in Collagen Antibody Induced Arthritis Mice. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072424
American Medical Association (AMA)
Dharmapatni, Anak A. S. S. K.& Cantley, Melissa D.& Marino, Victor& Perilli, Egon& Crotti, Tania N.& Smith, Malcolm D.…[et al.]. The X-Linked Inhibitor of Apoptosis Protein Inhibitor Embelin Suppresses Inflammation and Bone Erosion in Collagen Antibody Induced Arthritis Mice. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072424
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072424