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Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse
Joint Authors
Bondzio, Angelika
Einspanier, Ralf
Barton, Ann Kristin
Shety, Tarek
Gehlen, Heidrun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In chronic respiratory disease, matrix metalloproteinases (MMPs) contribute to pathological tissue destruction when expressed in excess, while tissue inhibitors of metalloproteinases (TIMPs) counteract MMPs with overexpression leading to fibrosis formation.
They may be out of balance in equine pneumopathies and serve as biomarkers of pulmonary inflammation.
We hypothesized that MMPs and TIMPs correlate to clinical findings and bronchoalveolar lavage fluid cytology in different equine chronic pneumopathies.
Using a scoring system, 61 horses were classified controls as free of respiratory disease (n=15), recurrent airway obstruction (RAO, n=17), inflammatory airway disease (IAD, n=18), or chronic interstitial pneumopathy (CIP, n=11).
Zymography and equine MMP and TIMP assays were used to detect MMP-2, MMP-8, MMP-9 as well as TIMP-1, and TIMP-2 in BALF supernatant.
MMP-2, TIMP-1, and TIMP-2 concentrations were significantly increased in RAO and IAD compared to controls.
MMP-9 concentration and MMP-8 activity evaluated by fluorimetry were significantly increased in RAO, IAD, and CIP.
These results were confirmed by zymography for MMP-2 and MMP-9 activity in 52 horses.
In conclusion, MMPs and TIMPs correlate well with clinical and cytologic findings.
These findings support the usefulness of MMPs, TIMPs, and their ratios to evaluate the severity of respiratory disease and may help to identify subclinical cases.
American Psychological Association (APA)
Barton, Ann Kristin& Shety, Tarek& Bondzio, Angelika& Einspanier, Ralf& Gehlen, Heidrun. 2015. Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072429
Modern Language Association (MLA)
Barton, Ann Kristin…[et al.]. Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072429
American Medical Association (AMA)
Barton, Ann Kristin& Shety, Tarek& Bondzio, Angelika& Einspanier, Ralf& Gehlen, Heidrun. Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072429
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072429