Carbon Monoxide Inhibits Tenascin-C Mediated Inflammation via IL-10 Expression in a Septic Mouse Model
Joint Authors
Li, Chun-shi
Zhang, Qinggao
Ryter, Stefan W.
Chen, Yingqing
Joe, Yeonsoo
Uddin, Md. Jamal
Chung, Hun Taeg
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Tenascin-C (TN-C), an extracellular matrix (ECM) glycoprotein, is specifically induced upon tissue injury and infection and during septic conditions.
Carbon monoxide (CO) gas is known to exert various anti-inflammatory effects in various inflammatory diseases.
However, the mechanisms underlying the effect of CO on TN-C-mediated inflammation are unknown.
In the present study, we found that treatment with LPS significantly enhanced TN-C expression in macrophages.
CO gas, or treatment with the CO-donor compound, CORM-2, dramatically reduced LPS-induced expression of TN-C and proinflammatory cytokines while significantly increased the expression of IL-10.
Treatment with TN-C siRNA significantly suppressed the effects of LPS on proinflammatory cytokines production.
TN-C siRNA did not affect the CORM-2-dependent increase of IL-10 expression.
In cells transfected with IL-10 siRNA, CORM-2 had no effect on the LPS-induced expression of TN-C and its downstream cytokines.
These data suggest that IL-10 mediates the inhibitory effect of CO on TN-C and the downstream production of proinflammatory cytokines.
Additionally, administration of CORM-2 dramatically reduced LPS-induced TN-C and proinflammatory cytokines production while expression of IL-10 was significantly increased.
In conclusion, CO regulated IL-10 expression and thus inhibited TN-C-mediated inflammation in vitro and in vivo.
American Psychological Association (APA)
Uddin, Md. Jamal& Li, Chun-shi& Joe, Yeonsoo& Chen, Yingqing& Zhang, Qinggao& Ryter, Stefan W.…[et al.]. 2015. Carbon Monoxide Inhibits Tenascin-C Mediated Inflammation via IL-10 Expression in a Septic Mouse Model. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1072454
Modern Language Association (MLA)
Uddin, Md. Jamal…[et al.]. Carbon Monoxide Inhibits Tenascin-C Mediated Inflammation via IL-10 Expression in a Septic Mouse Model. Mediators of Inflammation No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1072454
American Medical Association (AMA)
Uddin, Md. Jamal& Li, Chun-shi& Joe, Yeonsoo& Chen, Yingqing& Zhang, Qinggao& Ryter, Stefan W.…[et al.]. Carbon Monoxide Inhibits Tenascin-C Mediated Inflammation via IL-10 Expression in a Septic Mouse Model. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1072454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072454