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ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury
Joint Authors
Li, Jun
Han, Shichao
Cai, Weixia
Yang, Xuekang
Jia, Yanhui
Zheng, Zhao
Wang, Hongtao
Li, Yan
Gao, Jianxin
Fan, Lei
Hu, Dahai
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-20
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The NLRP3 inflammasome is necessary for initiating acute sterile inflammation.
However, its role in the pathogenesis of burn-induced acute lung injury (ALI) is unknown.
This study aimed to determine the role of the NLRP3 inflammasome and the signaling pathways involved in burn-induced ALI.
We observed that the rat lungs exhibited enhanced inflammasome activity after burn, as evidenced by increased levels of NLRP3 expression and Caspase-1 activity and augmented inflammatory cytokines.
Inhibition of NLRP3 inflammasome by BAY11-7082 attenuated burn-induced ALI, as demonstrated by the concomitant remission of histopathologic changes and the reduction of myeloperoxidase (MPO) activity, inflammatory cytokines in rat lung tissue, and protein concentrations in the bronchoalveolar lavage fluid (BALF).
In the in vitro experiments, we used AMs (alveolar macrophages) challenged with burn serum to mimic the postburn microenvironment and noted that the serum significantly upregulated NLRP3 inflammasome signaling and reactive oxygen species (ROS) production.
The use of ROS scavenger N-acetylcysteine (NAC) partially reversed NLRP3 inflammasome activity in cells exposed to burn serum.
These results indicate that the NLRP3 inflammasome plays an essential role in burn-induced ALI and that burn-induced NLRP3 inflammasome activity is a partly ROS-dependent process.
Targeting this axis may represent a promising therapeutic strategy for the treatment of burn-induced ALI.
American Psychological Association (APA)
Han, Shichao& Cai, Weixia& Yang, Xuekang& Jia, Yanhui& Zheng, Zhao& Wang, Hongtao…[et al.]. 2015. ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1072531
Modern Language Association (MLA)
Han, Shichao…[et al.]. ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury. Mediators of Inflammation No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1072531
American Medical Association (AMA)
Han, Shichao& Cai, Weixia& Yang, Xuekang& Jia, Yanhui& Zheng, Zhao& Wang, Hongtao…[et al.]. ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1072531
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072531