Gestation Related Gene Expression of the Endocannabinoid Pathway in Rat Placenta
Joint Authors
Rice, Gregory E.
Mitchell, Murray D.
Nitert, Marloes Dekker
Vaswani, Kanchan
Chan, Hsiu-Wen
Peiris, Hassendrini N.
Wood Bradley, Ryan J.
Armitage, James A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Mammalian placentation is a vital facet of the development of a healthy and viable offspring.
Throughout gestation the placenta changes to accommodate, provide for, and meet the demands of a growing fetus.
Gestational gene expression is a crucial part of placenta development.
The endocannabinoid pathway is activated in the placenta and decidual tissues throughout pregnancy and aberrant endocannabinoid signaling during the period of placental development has been associated with pregnancy disorders.
In this study, the gene expression of eight endocannabinoid system enzymes was investigated throughout gestation.
Rat placentae were obtained at E14.25, E15.25, E17.25, and E20, RNA was extracted, and microarray was performed.
Gene expression of enzymes Faah, Mgll, Plcd4, Pld1, Nat1, Daglα, and Ptgs2 was studied (cohort 1, microarray).
Biological replication of the results was performed by qPCR (cohort 2).
Four genes showed differential expression (Mgll, Plcd4, Ptgs2, and Pld1), from mid to late gestation.
Genes positively associated with gestational age were Ptgs2, Mgll, and Pld1, while Plcd4 was downregulated.
This is the first comprehensive study that has investigated endocannabinoid pathway gene expression during rat pregnancy.
This study provides the framework for future studies that investigate the role of endocannabinoid system during pregnancy.
American Psychological Association (APA)
Vaswani, Kanchan& Chan, Hsiu-Wen& Peiris, Hassendrini N.& Nitert, Marloes Dekker& Wood Bradley, Ryan J.& Armitage, James A.…[et al.]. 2015. Gestation Related Gene Expression of the Endocannabinoid Pathway in Rat Placenta. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072611
Modern Language Association (MLA)
Vaswani, Kanchan…[et al.]. Gestation Related Gene Expression of the Endocannabinoid Pathway in Rat Placenta. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072611
American Medical Association (AMA)
Vaswani, Kanchan& Chan, Hsiu-Wen& Peiris, Hassendrini N.& Nitert, Marloes Dekker& Wood Bradley, Ryan J.& Armitage, James A.…[et al.]. Gestation Related Gene Expression of the Endocannabinoid Pathway in Rat Placenta. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072611