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Upregulation of EMMPRIN (OX47) in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve Injury
Joint Authors
Wang, Qun
Sun, Yanyuan
Ren, Yingna
Gao, Yandong
Tian, Li
Liu, Yang
Pu, Yanan
Gou, Xingchun
Chen, Yanke
Lu, Yan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Matrix metalloproteinases (MMPs) are widely implicated in inflammation and tissue remodeling associated with various neurodegenerative diseases and play an important role in nociception and allodynia.
Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) plays a key regulatory role for MMP activities.
However, the role of EMMPRIN in the development of neuropathic pain is not clear.
Western blotting, real-time quantitative RT-PCR (qRT-PCR), and immunofluorescence were performed to determine the changes of messenger RNA and protein of EMMPRIN/OX47 and their cellular localization in the rat dorsal root ganglion (DRG) after nerve injury.
Paw withdrawal threshold test was examined to evaluate the pain behavior in spinal nerve ligation (SNL) model.
The lentivirus containing OX47 shRNA was injected into the DRG one day before SNL.
The expression level of both mRNA and protein of OX47 was markedly upregulated in ipsilateral DRG after SNL.
OX47 was mainly expressed in the extracellular matrix of DRG.
Administration of shRNA targeted against OX47 in vivo remarkably attenuated mechanical allodynia induced by SNL.
In conclusion, peripheral nerve injury induced upregulation of OX47 in the extracellular matrix of DRG.
RNA interference against OX47 significantly suppressed the expression of OX47 mRNA and the development of mechanical allodynia.
The altered expression of OX47 may contribute to the development of neuropathic pain after nerve injury.
American Psychological Association (APA)
Wang, Qun& Sun, Yanyuan& Ren, Yingna& Gao, Yandong& Tian, Li& Liu, Yang…[et al.]. 2015. Upregulation of EMMPRIN (OX47) in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve Injury. Neural Plasticity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075310
Modern Language Association (MLA)
Wang, Qun…[et al.]. Upregulation of EMMPRIN (OX47) in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve Injury. Neural Plasticity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075310
American Medical Association (AMA)
Wang, Qun& Sun, Yanyuan& Ren, Yingna& Gao, Yandong& Tian, Li& Liu, Yang…[et al.]. Upregulation of EMMPRIN (OX47) in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve Injury. Neural Plasticity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075310