CO2 Pneumoperitoneum Preserves β-Arrestin 2 Content and Reduces High Mobility Group Box-1 (HMGB-1) Expression in an Animal Model of Peritonitis
Joint Authors
Bitto, Alessandra
Romeo, Carmelo
Pizzino, Gabriele
Irrera, Natasha
Montalto, Angela Simona
Impellizzeri, Pietro
Costa, Gaetano
Minutoli, Letteria
Squadrito, Francesco
Altavilla, Domenica
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Laparoscopy (LS) has been shown to decrease the inflammatory sequelae of endotoxemia.
β-arrestin 2 plays an important function in signal transduction pathway of TLR4.
High mobility group box-1 (HMGB-1) is involved in the delayed systemic inflammatory response.
We investigated the effects of CO2 insufflation on liver, lung, and kidney expression of both β-arrestin 2 and HMGB-1 during sepsis.
Cecal ligation and puncture (CLP) was performed in male rats and 6 h later the animals were randomly assigned to receive a CO2 pneumoperitoneum or laparotomy.
Animals were euthanized; liver, lung, and kidney were removed for the evaluation of β-arrestin 2 and HMGB-1 expression.
Immunohistochemical detection of myeloperoxidase (MPO) was investigated in lung and liver and bacterial load was determined in the peritoneal fluid.
CO2 pneumoperitoneum reduced peritoneal bacterial load, increased the expression of β-arrestin 2, and blunted the expression of the potent proinflammatory HMGB-1 in liver, lung, and kidney compared with laparotomy.
Liver and lung MPO was markedly reduced in rats subjected to LS compared with laparotomy.
We believe that CO2 exerts an early protective effect by reducing bacterial load and likely toll-like receptor activation which in turn leads to a preserved β-arrestin 2 expression and a reduced HMGB-1 expression.
American Psychological Association (APA)
Montalto, Angela Simona& Bitto, Alessandra& Minutoli, Letteria& Impellizzeri, Pietro& Costa, Gaetano& Irrera, Natasha…[et al.]. 2015. CO2 Pneumoperitoneum Preserves β-Arrestin 2 Content and Reduces High Mobility Group Box-1 (HMGB-1) Expression in an Animal Model of Peritonitis. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1075539
Modern Language Association (MLA)
Montalto, Angela Simona…[et al.]. CO2 Pneumoperitoneum Preserves β-Arrestin 2 Content and Reduces High Mobility Group Box-1 (HMGB-1) Expression in an Animal Model of Peritonitis. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1075539
American Medical Association (AMA)
Montalto, Angela Simona& Bitto, Alessandra& Minutoli, Letteria& Impellizzeri, Pietro& Costa, Gaetano& Irrera, Natasha…[et al.]. CO2 Pneumoperitoneum Preserves β-Arrestin 2 Content and Reduces High Mobility Group Box-1 (HMGB-1) Expression in an Animal Model of Peritonitis. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1075539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075539