A YeastDrosophila Screen to Identify New Compounds Overcoming Frataxin Deficiency
Joint Authors
Seguin, Alexandra
Monnier, Véronique
Palandri, Amandine
Bihel, Frédéric
Rera, Michael
Schmitt, Martine
Camadro, Jean-Michel
Tricoire, Hervé
Lesuisse, Emmanuel
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Friedreich’s ataxia (FA) is a rare neurodegenerative disease which is very debilitating for the patients who progressively lose their autonomy.
The lack of efficient therapeutic treatment of the disease strongly argues for urgent need to search for new active compounds that may stop the progression of the disease or prevent the appearance of the symptoms when the genetic defect is diagnosed early enough.
In the present study, we used a yeast strain with a deletion of the frataxin homologue gene as a model of FA cells in a primary screen of two chemical libraries, a fraction of the French National Chemical Library (5500 compounds) and the Prestwick collection (880 compounds).
We ran a secondary screen on Drosophila melanogaster flies expressing reduced levels of frataxin during larval development.
Half of the compounds selected in yeast appeared to be active in flies in this developmental paradigm, and one of the two compounds with highest activities in this assay partially rescued the heart dilatation phenotype resulting from heart specific depletion of frataxin.
The unique complementarity of these two frataxin-deficient models, unicellular and multicellular, appears to be very efficient to select new compounds with improved selectivity, bringing significant perspectives towards improvements in FA therapy.
American Psychological Association (APA)
Seguin, Alexandra& Monnier, Véronique& Palandri, Amandine& Bihel, Frédéric& Rera, Michael& Schmitt, Martine…[et al.]. 2015. A YeastDrosophila Screen to Identify New Compounds Overcoming Frataxin Deficiency. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075692
Modern Language Association (MLA)
Seguin, Alexandra…[et al.]. A YeastDrosophila Screen to Identify New Compounds Overcoming Frataxin Deficiency. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075692
American Medical Association (AMA)
Seguin, Alexandra& Monnier, Véronique& Palandri, Amandine& Bihel, Frédéric& Rera, Michael& Schmitt, Martine…[et al.]. A YeastDrosophila Screen to Identify New Compounds Overcoming Frataxin Deficiency. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075692