Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca+2-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P)
Joint Authors
Ronchi, Juliana A.
Henning, Barbara
Ravagnani, Felipe G.
Figueira, Tiago R.
Castilho, Roger F.
dos Reis, Sergio F.
Vercesi, Anibal E.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In addition to be the cell’s powerhouse, mitochondria also contain a cell death machinery that includes highly regulated processes such as the membrane permeability transition pore (PTP) and reactive oxygen species (ROS) production.
In this context, the results presented here provide evidence that liver mitochondria isolated from Gracilinanus microtarsus, a small and short life span (one year) marsupial, when compared to mice, are much more susceptible to PTP opening in association with a poor NADPH dependent antioxidant capacity.
Liver mitochondria isolated from the marsupial are well coupled and take up Ca 2+ but exhibited a much lower Ca 2+ retention capacity than mouse mitochondria.
Although the known PTP inhibitors cyclosporin A, ADP, and ATP significantly increased the marsupial mitochondria capacity to retain Ca 2+ , their effects were much larger in mice than in marsupial mitochondria.
Both fluorescence and HPLC analysis of mitochondrial nicotinamide nucleotides showed that both content and state of reduction (mainly of NADPH) were lower in the marsupial mitochondria than in mice mitochondria despite the similarity in the activity of the glutathione peroxidase/reductase system.
Overall, these data suggest that PTP opening is an important event in processes of Ca 2+ signalling to cell death mediated by mitochondrial redox imbalance in G.
microtarsus.
American Psychological Association (APA)
Ronchi, Juliana A.& Henning, Barbara& Ravagnani, Felipe G.& Figueira, Tiago R.& Castilho, Roger F.& dos Reis, Sergio F.…[et al.]. 2015. Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca+2-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P). Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075818
Modern Language Association (MLA)
Ronchi, Juliana A.…[et al.]. Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca+2-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P). Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075818
American Medical Association (AMA)
Ronchi, Juliana A.& Henning, Barbara& Ravagnani, Felipe G.& Figueira, Tiago R.& Castilho, Roger F.& dos Reis, Sergio F.…[et al.]. Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca+2-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P). Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075818
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075818